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Toxicity to reproduction: other studies

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Administrative data

Endpoint:
toxicity to reproduction: other studies
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Publication in foreign language (chinese). Only the abstract is available in englisch.

Data source

Reference
Reference Type:
publication
Title:
Effect of Carbon Disulfide on Expression of Genes Related with Mitochondrion Mediated Sertoli-Germ Cells Apoptosis in Male Rats
Author:
Zhang Zhen, Wang Wei, Dong Yu, Huang Xiao-yu, Guo Yin-sheng, Zhou Yi-jun, Chen Guo-yuan
Year:
2013
Bibliographic source:
J Environ Occup Med, Vol. 30 (11): 870-874

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
A 22 -24 day old SD male rat of clean grade was selected to establish the rat sertoli-germ cells co-culture system in vitro. According to the designed concentrations of CS2 (0, 1, 2, 4 µmol/mL), the cells were divided into four groups accordingly and RNA were extracted. The mRNA expression levels of Cyt C, Apaf-1, Caspase-3, Caspase-9, Alf, EndoG, Smac, IAPS, Bax, Bcl-2 were determined.
Type of method:
in vitro

Test material

Constituent 1
Chemical structure
Reference substance name:
Carbon disulphide
EC Number:
200-843-6
EC Name:
Carbon disulphide
Cas Number:
75-15-0
Molecular formula:
CS2
IUPAC Name:
methanedithione

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Age at study initiation: 22-24 days


Administration / exposure

Route of administration:
other: rat sertoli-germ cells were exposed in vitro
Doses / concentrations
Remarks:
Doses / Concentrations:
0, 1, 2, 4 µmol/L
Basis:

Control animals:
other: untreated cells

Results and discussion

Effect levels

Dose descriptor:
NOAEL
Remarks on result:
not determinable
Remarks:
no NOAEL identified

Any other information on results incl. tables

Comparing with the control group, the levels of Cyt C, Smac, IAPS, Caspase-3, Bax, and Bax/Bcl-2 mRNA in the sertoli germ cells were increased, while the levels of Apaf-1, Alf, Bcl-2, and EndoG mRNA were decreased in CS2 exposure groups (P < 0.05).

Applicant's summary and conclusion

Conclusions:
In conclusion, CS2 exposure affects the expression of genes related with mitochondrial mediated apoptosis, and it propably relates to reproductive toxicity induced by CS2.
Executive summary:

The effects on carbon disulfide (CS2) on the expression of genes which are related with mitochondrion mediated sertoli-germ cells apoptosis were studied in male rats. A 22 -24 day old SD male rat of clean grade was selected to establish the rat sertoli-germ cells co-culture system in vitro. According to the designed concentrations of CS2 (0, 1, 2, 4 µmol/mL), the cells were divided into four groups accordingly and RNA were extracted. The mRNA expression levels of Cyt C, Apaf-1, Caspase-3, Caspase-9, Alf, EndoG, Smac, IAPS, Bax, Bcl-2 were detected by real-time fluorescent quantitative polymerase chain reaction (RT-PCR). Comparing with the control group, the levels of Cyt C, Smac, IAPS, Caspase-3, Bax, and Bax/Bcl-2 mRNA in the sertoli germ cells were increased, while the levels of Apaf-1, Alf, Bcl-2, and EndoG mRNA were decreased in CS2 exposure groups (P<0.05). In conclusion, CS2 exposure affects the expression of genes related with mitochondrial mediated apoptosis, and it propably relates to reproductive toxicity induced by CS2.