Carbon disulphide

Toxicity to reproduction: other studies

Administrative data

Endpoint:

toxicity to reproduction: other studies

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Publication in foreign language (chinese). Only the abstract is available in englisch.

Data source

Reference

Reference Type:

publication

Title:

Effect of Carbon Disulfide on Expression of Genes Related with Mitochondrion Mediated Sertoli-Germ Cells Apoptosis in Male Rats

Author:

Zhang Zhen, Wang Wei, Dong Yu, Huang Xiao-yu, Guo Yin-sheng, Zhou Yi-jun, Chen Guo-yuan

Year:

2013

Bibliographic source:

J Environ Occup Med, Vol. 30 (11): 870-874

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

A 22 -24 day old SD male rat of clean grade was selected to establish the rat sertoli-germ cells co-culture system in vitro. According to the designed concentrations of CS2 (0, 1, 2, 4 µmol/mL), the cells were divided into four groups accordingly and RNA were extracted. The mRNA expression levels of Cyt C, Apaf-1, Caspase-3, Caspase-9, Alf, EndoG, Smac, IAPS, Bax, Bcl-2 were determined.

Type of method:

in vitro

Test material

Test material information

Constituent 1

Reference substance name:

Carbon disulphide

EC Number:

200-843-6

EC Name:

Carbon disulphide

Cas Number:

75-15-0

Molecular formula:

CS2

IUPAC Name:

methanedithione

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 22-24 days

Administration / exposure

Route of administration:

other: rat sertoli-germ cells were exposed in vitro

Doses / concentrations

Remarks:

Doses / Concentrations:
0, 1, 2, 4 µmol/L
Basis:

Control animals:

other: untreated cells

Results and discussion

Effect levels

Dose descriptor:

NOAEL

Remarks on result:

not determinable

Remarks:

no NOAEL identified

Any other information on results incl. tables

Comparing with the control group, the levels of Cyt C, Smac, IAPS, Caspase-3, Bax, and Bax/Bcl-2 mRNA in the sertoli germ cells were increased, while the levels of Apaf-1, Alf, Bcl-2, and EndoG mRNA were decreased in CS2 exposure groups (P < 0.05).

Applicant's summary and conclusion

Conclusions:

In conclusion, CS2 exposure affects the expression of genes related with mitochondrial mediated apoptosis, and it propably relates to reproductive toxicity induced by CS2.

Executive summary:

The effects on carbon disulfide (CS2) on the expression of genes which are related with mitochondrion mediated sertoli-germ cells apoptosis were studied in male rats. A 22 -24 day old SD male rat of clean grade was selected to establish the rat sertoli-germ cells co-culture system in vitro. According to the designed concentrations of CS2 (0, 1, 2, 4 µmol/mL), the cells were divided into four groups accordingly and RNA were extracted. The mRNA expression levels of Cyt C, Apaf-1, Caspase-3, Caspase-9, Alf, EndoG, Smac, IAPS, Bax, Bcl-2 were detected by real-time fluorescent quantitative polymerase chain reaction (RT-PCR). Comparing with the control group, the levels of Cyt C, Smac, IAPS, Caspase-3, Bax, and Bax/Bcl-2 mRNA in the sertoli germ cells were increased, while the levels of Apaf-1, Alf, Bcl-2, and EndoG mRNA were decreased in CS2 exposure groups (P<0.05). In conclusion, CS2 exposure affects the expression of genes related with mitochondrial mediated apoptosis, and it propably relates to reproductive toxicity induced by CS2.