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EC number: 200-843-6
CAS number: 75-15-0
Results after 10 Minutes Incubation Time
Opacity value = Difference (t130-t0) of Opacity
Permeability at 490 nm (OD490)*
In vitro score
Mean in vitro score
Proposed in vitro irritation Scale
Non eye irritant
Severe eye irritant
This in vitro study
was performed to assess the corneal irritation and damage
potential of carbon
disulfide by means of the BCOP assay
using fresh bovine corneas.
After a first opacity measurement of the fresh bovine
corneae (t0), carbon disulfide, the positive, and the
negative controls were applied to corneas and incubated for 10 minutes
at 32±2 °C in
complete medium. After the incubation phase the test item, the positive,
and the negative controls were each rinsed off the corneas and opacity
was measured again (t10). Further, the corneas were incubated
for another 120 minutes at 32±2
°C in complete medium, and opacity was measured a
third time (t130). After the opacity measurements
permeability of the corneas was determined while application of 1 mL of
a fluorescein solution for 90 minutes at 32±2
°C in a horizontal position. The liquid coming out was
With the negative
control (0.9% NaCl solution) neither an increase of opacity nor
permeability of the corneae could be observed. The mean in vitro score
was calculated as 0.38. The positive
control (2-Ethoxyethanol) showed clear opacity and distinctive
permeability of the corneae and therefore, is classified as severe eye
irritant. The mean in vitro score was calculated as 77.34. The
test item carbon disulfide did not cause any opacity or permeability of
the corneae compared with the results of the negative control. The
calculated mean in vitro score was 0.37 and therefore, the test
item was classified as non eye irritant.
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