Carbon disulphide

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to: OECD Guideline for the Testing of Chemicals, Draft Proposal for a New Guideline, In Vitro Skin Irritation: Reconstructed Human Epidermis (RhE) Test Method, 11 December 2009, Vers. 4.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD Guideline for the Testing of Chemicals, Draft Proposal for a New Guideline, In Vitro Skin Irritation: Reconstructed Human Epidermis (RhE) Test Method, 11 December 2009, Vers. 4.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Carbon disulphide

EC Number:

200-843-6

EC Name:

Carbon disulphide

Cas Number:

75-15-0

Molecular formula:

CS2

IUPAC Name:

methanedithione

Details on test material:

Identification: Carbon disulfide
Source: Supplied by Akzo Nobel
EC Number: 200-843-6
CAS Number: 75-15-0
Batch Number: 0600809041160
Molecular Weight: 76.1 g/mol
Purity: > 99 % (dose calculation not adjusted to purity) (Certificate of analysis provided by the supplier)
Stability in Solvent: Not indicated by the sponsor
Expiry Date: September 19, 2011
Storage Conditions: At room temperature, under Nitrogen, avoid vapour release and contact of vapour with open flame and/or hot surfaces.
Storage Conditions at Harlan Laboratories: At room temperature at about 20 °C, N2

Test system

Type of coverage:

other: in vitro testing

Amount / concentration applied:

Application volume: 15 µL per skin tissue
Test concentration: undiluted

Duration of treatment / exposure:

15 minutes treatment followed by 42 hours further incubation without test item

Details on study design:

One experiment was performed. Three skin tissues (EpiSkin TM Tissues) were treated with each 15 µL of the undiluted test item. The tissues were exposed to the test item for 15 minutes. Afterwards the test item was washed off the tissues and they were incubated for further 42 hours. The evaluation was done via MTT conversion.
Negative control: deionised water (15 µL applied to each of the triplicate of tissues)
Positive control: 5% SLS (Sodium lauryl sulphate) solution in deionised water (15 µL applied to each of the triplicate of tissues)

Results and discussion

In vitro

Results

Irritation / corrosion parameter:

other: relative absorbance value (%)

Value:

67

Vehicle controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other:

Remarks:

all 3 tissues. Time point: 15 min. Remarks: see below for details

Other effects / acceptance of results:

After treatment with carbon disulfide the relative absorbance values were decreased to 67.0%. This value is well above the threshold for irritancy of 50%. Therefore, the test item is not considered to possess an irritant potential.

Any other information on results incl. tables

Table 1: Results after treatment with carbon disulfide

 

Dose group	Treatment Interval	Absorbance 570 nm Tissue 1*	Absorbance 570 nm Tissue 2*	Absorbance 570 nm Tissue 3*	Mean Absorbance of 3 Tissues	Standard Deviation	Rel. Absorbance [% of Negative Control]**
Negative Control	15 min	0.667	0.687	0.662	0.672	2.0	100.0
Positive Control	15 min	0.341	0.176	0.294	0.270	12.6	40.2
Carbon disulfide	15 min	0.460	0.498	0.393	0.450	7.9	67.0

*       Mean of three replicate wells after blank correction
**      relative absorbance [rounded values]

Optical evaluation of the MTT-reducing capacity of the test item after 1 hour incubation with MTT-reagent did not show blue colour.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Remarks:

Migrated information Criteria used for interpretation of results: OECD GHS

Conclusions:

In conclusion, it can be stated that in this study and under the experimental conditions reported, the test item carbon disulfide is non irritant to skin.

Executive summary:

This in vitro study was performed to assess the irritation potential of Carbon disulfide by means of the Human Skin Model Test. Three tissues of the human skin model EpiSkin™ were treated with the test item, the negative or the positive control for 15 minutes. 15 µL of the test item were applied to each tissue, spread to match the tissue size. The test item, the positive and negative controls were washed off the skin tissues after 15 minutes treatment. After further incubation for about 42 hours the tissues were treated with the MTT solution for 3 hours following approximately 70 hours extraction of the colorant from the cells. The amount of extracted colorant wasdetermined photometrically at 570 nm. 15 µL of either the negative control (deionised water) or the positive control (5% Sodium lauryl sulfate) were applied to each tissue. After treatment with the negative control the absorbance values were well above the required acceptability criterion of mean 0.6≤OD≤1.5 for the15 minutestreatment interval thus showing the quality of the tissues. Treatment with the positive control induced a decrease in the relative absorbance as compared to the negative control to 40.2%. The standard deviations between the % variabilities of the test item, the positive and negative controls were below 8% (threshold of the "OECD Guideline for the Testing of Chemicals, Draft Proposal for a New Guideline,In Vitro Skin Irritation": 18%), thus ensuring the validity of the study. After treatment with the test item Carbon disulfide the relative absorbance values were decreased to 67.0%. This value is well above the threshold for irritancy of 50%. Therefore, the test item is not considered to possess an irritant potential.