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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

toxicity to other aquatic vertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles.

Data source

Referenceopen allclose all

Reference Type:
Toxicity and teratogenic effects of Carbon disulphide in frog embryo
Ghate HV
Bibliographic source:
Rivista di Biologia Vol. 78
Reference Type:
secondary source
Carbon disulfide
GDCh-Advisory Committee on Existing Chemicals of Environmental Relevance (BUA)
Bibliographic source:
BUA report 83

Materials and methods

Principles of method if other than guideline:
Effects of different concentrations of carbon disulphide on the embryonic development of frog investigated.
GLP compliance:

Test material

Constituent 1
Chemical structure
Reference substance name:
Carbon disulphide
EC Number:
EC Name:
Carbon disulphide
Cas Number:
Molecular formula:

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Details on test solutions:
CS2 was dissolved in alcohol (1 mL CS2 in 50 mL alcohol). This solution was used to prepare various concentrations of CS2 in aged tap water. Controls received appropiate quantity of alcohol.

Test organisms

Test organisms (species):
Microhyla ornata
Details on test organisms:
Fresh, naturally fertilized eggs of the frog Microhyla ornata were obtained from a local, unpolluted pond. The jelly was removed with forceps and the eggs were transferred to aged tapwater. Eggs in early gastrulation (dorsal lip) were then used in experimentation as per the procedure described earlier (Ghate, 1980 & 1983 a). 50 Embryos per concentration expodes.

Study design

Test type:
not specified
Water media type:
Limit test:

Results and discussion

Details on results:
The embryonic development of frogs was disturbed at a concentration of 126 mg CS2/L, with no hatching taking place (100% mortality) .

Any other information on results incl. tables

4 µL CS2 / 100 mL aged tapwater: no mortality / Development normal

8 µL CS2 / 100 mL aged tapwater: no mortality / Hatching delayed by few hours / Tadpoles showed slightly abnormal notochord

12 µL CS2 / 100 mL aged tapwater: no mortality / Hatching delayed 50% failed to hatch even at 96 hrs. / All were oedematic with wavy notochord

16 µL CS2 / 100 mL aged tapwater: 90% mortality in 24 hrs. / All dead in 48 hrs.

20 µL CS2 / 100 mL aged tapwater: 100% mortality in 24 hrs.

Applicant's summary and conclusion