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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline test but no data on GLP

Data source

Reference
Reference Type:
publication
Title:
Mutagenicity testing in the Salmonella typhimurium assay of phenolic compounds and phenolic fractios obtained from smokehouse smoke condensates
Author:
Pool BL and PZ Lin
Year:
1982
Bibliographic source:
Fd. Chem. Toxicol. 20, 383-391

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
o-cresol
EC Number:
202-423-8
EC Name:
o-cresol
Cas Number:
95-48-7
Molecular formula:
C7H8O
IUPAC Name:
o-cresol
Details on test material:
Test substance: o-cresol, purity: 98 %

Method

Species / strain
Species / strain / cell type:
other: Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538
Additional strain / cell type characteristics:
other: Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538
Metabolic activation:
with and without
Metabolic activation system:
A 9000 x g supernatant from Sprague-Dawley rat liver pretreated with Aroclor 1254.
Test concentrations with justification for top dose:
0, 5, 50, 500, 5000 µg/plate dissolved in DMSO
Vehicle / solvent:
DMSO
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
yes
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
other: Sodium azide, 2-nitrofluorene, 9-Aminoacridine, 2-Aminoanthracene
Details on test system and experimental conditions:
Ames test: plate incorporation methodology
Evaluation criteria:
A dose-related significantly increased number of revertants was evaluated as a positive result.
Statistics:
Joncheere test

Results and discussion

Test results
Species / strain:
other: Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
other: 5000 µg/plate
Vehicle controls validity:
valid
Untreated negative controls validity:
valid
Positive controls validity:
valid
Additional information on results:
Positive controls were functional.

Any other information on results incl. tables

o-cresol yielded a negative result in the Ames test performed according to OECD TG 471 in the presence and in the absence of an additional metabolic activation system (Pool 1982).

Applicant's summary and conclusion

Executive summary:

o-cresol yielded a negative result in the Ames test performed according to OEDCD TG 471 in the presence and in the absence of an additional metabolic activation system.