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EC number: 202-423-8 | CAS number: 95-48-7
- Life Cycle description
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- Endpoint summary
- Stability
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- Transport and distribution
- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
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- Specific investigations
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- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: DEV L9
- Principles of method if other than guideline:
- cell multiplication inhibition test
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- a stock solution was prepared; to achive the test concentrations a defined amount of the stock solution was dissolved
- Test organisms (species):
- Microcystis aeruginosa
- Details on test organisms:
- blue-green algae = cyanobacteria
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 8 d
- Test temperature:
- 27°C
- pH:
- initial pH 7.0
- Details on test conditions:
- -stock solution for test medium: 248 mg NaNO3, 19.5 mg K2HPO4, 750 mg MgSO4 x 7 H2O, 360 mg CaCl2 x 2 H2O,
400 mg NaSiO3, 580 mg Na2CO3, 30 mg citric acid, 30 mg ferric (III) citrate, 100 mg Na2EDTA x 2 H2O dissolved in 1 l double-distilled water containing the trace elements as H3BO3, MnCl2, ZnSO4, CuSO4, Na2MoO4 and CoCl2.
-incubation of 10 ml test solution
-the experiment was carried out in duplicate dilution series
-the concentration of the algal suspension was measured turbidimetrically - Duration:
- 8 d
- Dose descriptor:
- other: TGK
- Effect conc.:
- 6.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: cell multiplication
- Details on results:
- TGK = Toxicity threshold, determined at 1% effect compared to control
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The toxicity of o-cresol to the blue-green algae Microcystis aeruginosa (Cyanaophyceae) was determined in a cell multiplication inhibition test according to the guideline DEV L9. The relevant 8d-NOEC (EC3) is 6.8 mg/L.
- Executive summary:
The toxicity of o-cresol to the blue-green algae Microcystis aeruginosa (Cyanaophyceae) was determined in a cell multiplication inhibition test according to the guideline DEV L9. Test conditions were as follows: continuous light, 27°C, with or without neutralization. The pH of the neutralized test solution was 7. For the endpoint biomass at the end of the test an 8 day-toxicity threshold value of 6.8 mg/l was obtained (nominal concentration). This toxicological threshold can also be regarded as a NOEC- or an EC3-value. For this reason the NOEC value can be given as 6.8 mg/l.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure in accordance with national standard methods with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: NEN-6506 (1980)
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Test organisms (species):
- Selenastrum sp.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 26 +/- 3°C
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 75 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- not specified
- Conclusions:
- With a growth inhibition test according to NEN-6506 (1980) on alga Selenastrum sp. a 96-hour EC50 of 100 mg/L based on nominal concentrations was determined for o-cresol.
- Executive summary:
With a growth inhibition test according to NEN-6506 (1980) on alga Selenastrum sp. a 96-hour EC50 of 100 mg/L based on nominal concentrations was determined for o-cresol.
Referenceopen allclose all
In the cited literature, the experiments were conducted with the species Microcystis aeruginosa and Scenedesmus quadricauda. In the OECD/ICCA HPV programm it was stated that it is unclear whether the algae S. quadricauda are within the exponential growth throughout the whole exposure period of 8 days. Accordingly, the study was assessed to be not reliable (reliability 3). However, the bluegreen algae Microcystis aeruginosa have a lower rate of reproduction compared to green algae and are still in the exponential growth rate under the experimental conditions after 8 d.
Description of key information
The toxicity of o-cresol to the blue-green algae Microcystis aeruginosa (Cyanaophyceae) was determined in a cell multiplication inhibition test according to the guideline DEV L9. The relevant 8d-NOEC (EC3) is 6.8 mg/L .
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 6.8 mg/L
Additional information
Concerning toxicity to aquatic algae there are several tests towards various species using o-cresol as test substance available.
The lowest toxicity value obtained with a blue-green algae is taken into account for further assessment.
The toxicity of o-cresol to the blue-green algae Microcystis aeruginosa (Cyanaophyceae) was determined in a cell multiplication inhibition test according to the guideline DEV L9. Test conditions were as follows: continuous light, 27°C, with or without neutralization. The pH of the neutralized test solution was 7. For the endpoint biomass at the end of the test an 8 day-toxicity threshold value of 6.8 mg/l was obtained (nominal concentration). This toxicological threshold can also be regarded as a NOEC- or an EC3-value. For this reason the NOEC value can be given as 6.8 mg/l.
With a growth inhibition test according to a dutch national standard method NEN-6506 (1980) on alga Selenastrum sp. a 96-hour EC50 of 100 mg/L based on nominal concentrations was determined for o-cresol.
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