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EC number: 603-837-5
CAS number: 134605-64-4
EC50 > 100 mg/L, activated sludge, OECD 209, EEC L133 s. 118-122, Grade 1996
The effect of the test material on the respiration rate of activated
sludge was investigated in line with GLP and in accordance with the
standardised guidelines OECD 209 and EEC L133 s. 118-122 (30 May 1988).
Activated sludge was exposed to nominal concentrations of the test
material at 1.0, 3.2, 10, 32 and 100 mg/L. Blank controls and
3,5-dichlorophenol as a positive reference substance were run
concurrently for comparison.
Under the conditions of the test no inhibition was observed within the
concentration range tested. The EC50 was therefore > 100 mg/L, the
highest concentration tested. It is considered that the test material is
not toxic to bacteria. The EC50 obtained from the reference substance
(EC50 = 16.5 mg/L) and the respiration rates of the blank
controls were within the acceptable range and fulfilled the validity
The study was performed according to GLP, in line with standardised
guidelines. Accordingly the study has been assigned a reliability score
of 1 in line with the principles for assessing data quality set out by
Klimisch (1997). The study is considered to be reliable, relevant and
adequate for the purposes of risk assessment.
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