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EC number: 603-837-5
CAS number: 134605-64-4
Freshwater: IC50 = 11 µg a.i./L, NOAEC = 1.6 µg a.i./L, Lemna gibba, EPA 540/9-82-020, Thompson & Swigert 1997b
The toxic effects of the test material to aquatic plant species was
determined in a 14-day toxicity study performed on Lemna gibba.
The study was conducted according to GLP and in line with the
standardised guideline EPA 540/9-82-020. L. gibba were exposed to
the test material at initial measured concentrations of 0.40, 0.81, 1.6,
3.2, 6.1, 14 and 26 µg a.i./L under static freshwater conditions. The
test solutions were analysed by HPLC, due to a decline in test material
concentration over the exposure period, the results were expressed
relative to Day 0 concentrations. Each concentration was tested in
triplicate with negative and solvent controls run concurrently for
comparison. Five plants totalling 15 fronds were used to initiate each
replicate. Direct counts of frond numbers were performed on Days 3, 6,
9, 12 and 14. Cultures were also monitored for chlorosis, necrosis,
break-up, root destruction, death and any other abnormalities.
Under the conditions of the test signs of toxicity were observed at
concentrations ≥ 3.2 µg a.i./L. The most sensitive parameters were
necrotic and dead fronds, frond size and root destruction, where plants
were significantly (p<0.05) affected at concentrations ≥ 3.2 µg a.i./L.
Significant (p<0.05) reductions in frond and plant production in
comparison to the pooled controls were observed at concentrations ≥ 6.1
and ≥ 26 µg a.i./L, respectively. Break up was first observed on Day 9
of exposure in the 14 µg a.i./L treatment group and by Day 6 in the 26
µg a.i./L group. There was no statistical (p>0.05) difference in
chlorotic fronds, in comparison to the pooled controls, at any of the
Based on these findings the IC50 was determined to be 11 µg a.i./L with
95% confidence limits of 8.7 and 13 µg a.i./L; the NOAEC was determined
to be 1.6 µg a.i./L.
The study was performed to a high standard, according to GLP and a
standardised guideline. Accordingly the study has been assigned a
reliability score of 1 in line with the principles for assessing data
quality set out by Klimisch (1997). The available data are deemed to be
relevant, reliable and adequate for the purposes of risk assessment.
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