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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 1982
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report Date:
1982

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Deviations:
yes
Remarks:
Analytical purity not reported. No GLP. Observation time too short
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,2,3- trichlorobenzene (solidified melted)
- Physical state: solid
-Analytical purity: no data

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Hacking & Churchill ltd., Huntingdon, UK
- Weight at study initiation: 3 kg
- Diet (e.g. ad libitum): Futter "Ssniff K4" (Ssniff Versuchstierdiaten GmbH, Soest/Westf., Germany) ad libitum
- Water (e.g. ad libitum): ad libitum



ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-21°C Celsius
- Humidity (%): 40-50%
- Photoperiod (hrs dark / hrs light): 12 hrs dark / 12 hrs ligh (7 a.m.-7 p.m.)


IN-LIFE DATES: The test was conducted during April 1982

Test system

Type of coverage:
semiocclusive
Preparation of test site:
shaved
Vehicle:
other: test subtance was prepared as a paste with water
Controls:
other: the untreated site served as control
Amount / concentration applied:
Concentration: 500 mg
Duration of treatment / exposure:
4 hour(s)
Observation period:
1, 24, 48 and 72 hours, 7 days
Number of animals:
3
Details on study design:
The flank skin of the three animals was shaved on both sides with electric cutter on an area of about 6x6 cm (24 h before testing the intact skin)

0.5 g of the substance mixed with water was applied on a gauze patch (2.5x2.5 cm).


The exposure time lasted 4h, then the patches were removed and the exposed area was washed with water.




SCORING SYSTEM: accoring to DRAIZE

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
animal #1
Time point:
other: 24, 48, and 72 hours
Score:
0.3
Max. score:
4
Reversibility:
fully reversible
Irritation parameter:
erythema score
Basis:
animal #2
Time point:
other: 24, 48, and 72 hours
Score:
0.3
Max. score:
4
Irritation parameter:
erythema score
Basis:
animal #3
Time point:
other: 24, 48, and 72 hours
Score:
0
Max. score:
4
Irritation parameter:
edema score
Basis:
animal #1
Time point:
other: 24, 48, and 72 hours
Score:
0
Max. score:
4
Irritation parameter:
edema score
Basis:
animal #2
Time point:
other: 24, 48, and 72 hours
Score:
0
Max. score:
4
Irritation parameter:
edema score
Basis:
animal #3
Time point:
other: 24, 48, and 72 hours
Score:
0
Max. score:
4
Irritant / corrosive response data:
Additional symptoms were not observed

Any other information on results incl. tables

Evaluation results of skin reactions after 1,2,3-trichlorobenzene administration to rabbits

Readings

 Animal

 Erythema

 Edema

 24 h

 68

 0

 0

 24 h

 69

 0

 0

 24 h

 70

 1

 0

 48 h

 68

 0

 0

 48 h

 69

 0

 0

 48 h

 70

 1

 0

 72 h

 68

 0

 0

 72 h

 69

 0

 0

 72h

 70

 0

 0

 Mean24 -72h

 68

 0.3

 0

 Mean24 -72h

 69

 0.3

 0

 Mean24 -72h

 70

 0

 0

Total mean

 

 0.2

 0

 

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Executive summary:

Suberg H (1982)

An acute dermal irritation/corrosion study was conducted according OECD Guideline 404 with deviations (Analytical purity not reported. No GLP. Observation time too short).

3 New Zealand White rabbits were exposed to 0.5 g 1,2,3 -trichlorobenzene for 24 hours. Observation time points were 1h, 24h, 48, 72h and 7 days after removal of the patch.

The mean erythema score at 24-72h after exposure was 0.3 for 2/3 of the animals tested. The oedema score between was found to have a value of 0 at 24 -72h after exposure.

On the basis of the data obtained 1,2,3-trichlorobenzene can not be classified as irritant.