Registration Dossier

Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Phytotoxicity studies with Lactuca sativa in soil and nutrient solution
Author:
Hulzebos EM, Adema DMM, Dirven-van Breemen, Henzen L, van Dis, WA, Herbold, HA, Hoekstra JA, Baerselman, R, van Gestel CAM
Year:
1993
Bibliographic source:
Environ. Toxicol. Chem. 12 (6): 1079-1094

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
Deviations:
no
Principles of method if other than guideline:
Additional semistatic experiments with the nutrient solutions were carried out
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
purity: >= 95%

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- soil: analytical monitoring took place at the start and at the end of the test
- nutrient solution: analytical monitoring took place at the start of the test and just before the next replacement of the test solution

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- all chlorobenzenes were either dissolved in acetone and/or mixed with a small amount of quartz sand
- the compound was mixed through the soil using the quartz sand as a carrier

Study design

Test type:
early seedling growth toxicity test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
no
Remarks:
soil: 14 days, nutrient solution: 16 d

Test conditions

Test temperature:
21+/-4 °C
pH:
7.5
Moisture:
soil experiment: 25-30% moisture content (80% of the water-holding capacity)
Details on test conditions:
TEST SYSTEM
- Testing facilities:
a) National Institute of Public Health and Environmental Protection (RIVM), Bilthoven, NL
b) Institute of Environmental Science (TNO), Delft, NL
- Test container (type, material, size): 0.25-L plastic trays
- Amount of soil: 400g
- No. of seeds per container: 10
- No. of plants (retained after thinning): 5
- No. of replicates per treatment group: 2 (for control and for at least three test concentrations)

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: no data
- Collection procedures: no data
- Soil texture (if natural soil)
- % clay: RIVM: 24; TNO: 12
- % organic matter content: RIVM: 1.8; TNO: 1.4
- Pretreatment of soil: sieved 4-mm before test start
- Storage (condition, duration): no data

NUTRIENT MEDIUM (if used)
- according to Steiner (1968)

GROWTH CONDITIONS
- Photoperiod: 16:8 h light:dark
- Light source: fluorescent tubes
- Light intensity and quality: 6,500 lx
- Relative humidity (%): 40-80
- other: trays were covered with glass plates until germination of seedlings
- Watering regime and schedules:
- Water source/type: demineralized water
- Volume applied: no data
- Interval of applications: daily
- Method of application: no data
Nominal and measured concentrations:
soil experiment: nominal concentrations in µg/g dry soil were spaced by a factor of 3.2,
nutrient solution experiment: nominal concentrations in mg/L were spaced by a factor of 3.2

Results and discussion

Effect concentrationsopen allclose all
Species:
Lactuca sativa
Duration:
7 d
Dose descriptor:
EC50
Effect conc.:
> 1 - < 3.2 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: result from RIVM-test facility
Species:
Lactuca sativa
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
1 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: 95% CL: 0.2-5, result from RIVM-test facility
Species:
Lactuca sativa
Duration:
7 d
Dose descriptor:
EC50
Effect conc.:
5.8 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: 95% CL: 4.5-7.4, result from TNO-test facility
Species:
Lactuca sativa
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
3.8 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: 95% CL: 3.4-4.2; result from TNO-test facility
Species:
Lactuca sativa
Duration:
16 d
Dose descriptor:
EC50
Effect conc.:
0.028 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: 95% CL: 0.022-0.036, nutrient solution, result from TNO-test facility

Any other information on results incl. tables

Whereas the presented tests were not carried out according to the EU-TGD the results need to be recalculated for a standard soil that contains 3.4% organic matter:

 

Reported (mg/kg dw)

Recalculated (mg/kg dw)

Lactuca sativa : 7-day EC50; RIVM

>1 - <3.2

>1.9 - <6.0

Lactuca sativa : 14-day EC50; RIVM

1 (0.2-5)

1.9 (0.4-9.4)

Lactuca sativa : 7-day EC50; TNO

5.8 (4.5-7.4)

14.1 (10.9-18)

Lactuca sativa : 14-day EC50; TNO

3.8 (3.4-4.2)

9.2 (8.3-10.2)

Applicant's summary and conclusion