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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From: 12.1.1982 To: 2.2.1982
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with accepatable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report Date:
1982

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
yes
Remarks:
No analytical purity reported. No GLP. Only 2 dosis tested.
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,2,3-trichlorobenzene solidified melted
- Physical state: solid
- Analytical purity: not reported

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Sparte, OC, Germany
- Age at study initiation: 9-14 weeks old
- Weight at study initiation: 182 (males), 177 (females)
- Fasting period before study: from 16 hours before the application to 4 hours after the application
- Housing: Makrolon cages type III
- Diet (e.g. ad libitum): ad libitum (except during the fasting period)
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 1.5 ° C
- Humidity (%): 60 ± 5%
- Photoperiod (hrs dark / hrs light): 12 hrs dark / 12 hrs light (7 a.m-7 p.m)


IN-LIFE DATES: From: 12.1.1982 To: 2.2.1982

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: Lutrol
Details on oral exposure:
MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg bw


Doses:
3100 and 5000 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
other: not applicable
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: At the day of the treatment the animals were controlled several times. During the 14-day observation period they were monitored twice per day ( once per day over weekends and holidays). The body weights were recorded at the day of the treatment, after 1 week and the end of the observation period.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, gross pathology

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 5 000 mg/kg bw
Remarks on result:
other: 95% CL: Not applicable
Mortality:
No mortality was observed in the male and female group dosed with 3100  mg/kg bw 2/5 males and 2/5 females dosed with 5000 mg/kg bw died within the first  week post application.
Clinical signs:
Rats of both sex and in both dose groups suffered from weight loss, rough  fur, bloody snout and reduced general condition.
Body weight:
weight loss was reported
Gross pathology:
Gross autopsy of some of the surviving rats showed no pathological  findings.

Any other information on results incl. tables

Dosis

Toxicologicalresults

Time ofdeath

Start ofthesymptoms

mL/kg

Deadanimals

Animalswithsymptoms

Total ofanimalemployed

 

 

Males

3.1

0

3

5

 

7 d

5.0

2

5

5

2-3d

24 h

Females

3.1

0

4

5

 

 

5.0

2

5

5

2-6 d

24 h

 

 

Table 1.Effect of acute oral toxicity test

Applicant's summary and conclusion

Executive summary:

Löser E (1982)

In order to test the acute oral toxicity of 1,2,3 -trichlorobenzene, 5 male and 5 female tested were dosed by gavage (3100 and 5000 mg/kg bw) with a method similar to OECD guideline 401 with deviations (No analytical purity reported. No GLP. Only 2 dosis tested)

Tthe following toxicological effects were observed:

1) Mortality: no mortality was observed in the male and female group dosed with 3100  mg/kg bw 2/5 males and 2/5 females dosed with 5000 mg/kg bw died within the first  week post application.

2) Signs of intoxication
Rats of both sex and in both dose groups suffered from weight loss, rough  fur, bloody snout and reduced general condition.

3) Gross autopsy 
Gross autopsy of some of the surviving rats showed no pathological  findings.