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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From: 12.1.1982 To: 2.2.1982
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1982

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
yes
Remarks:
No analytical purity reported. No GLP. Only 2 doses tested.
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1,2,3-trichlorobenzene
EC Number:
201-757-1
EC Name:
1,2,3-trichlorobenzene
Cas Number:
87-61-6
Molecular formula:
C6H3Cl3
IUPAC Name:
1,2,3-trichlorobenzene
Test material form:
solid
Details on test material:
- Name of test material (as cited in study report): 1,2,3-trichlorobenzene solidified melted
- Physical state: solid
- Analytical purity: not reported
Specific details on test material used for the study:
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not reported
- Stability and homogeneity of the test material in the vehicle/solvent under test conditions (e.g. in the exposure medium) and during storage: not reported
- Stability in the medium, i.e. sensitivity of the test material to hydrolysis and/or photolysis: not reported
- Solubility and stability of the test material in the solvent/vehicle and the exposure medium: not reported
- Reactivity of the test material with the incubation material used (e.g. plastic ware): not reported

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing (e.g. warming, grinding): formulated in Lutrol
- Final concentration of a dissolved solid, stock liquid or gel: not reported

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Sparte, OC, Germany
- Age at study initiation: 9-14 weeks old
- Weight at study initiation: 182 (males), 177 (females)
- Fasting period before study: from 16 hours before the application to 4 hours after the application
- Housing: Makrolon cages type III
- Diet (e.g. ad libitum): ad libitum (except during the fasting period)
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 1.5 ° C
- Humidity (%): 60 ± 5%
- Photoperiod (hrs dark / hrs light): 12 hrs dark / 12 hrs light (7 a.m-7 p.m)


IN-LIFE DATES: From: 12.1.1982 To: 2.2.1982

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: Lutrol
Details on oral exposure:
VEHICLE
- Concentration in vehicle: not reported
- Amount of vehicle (if gavage): not reported
- Justification for choice of vehicle: not reported
- Lot/batch no. (if required): not reported
- Purity: not reported

MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg bw
Doses:
3100 and 5000 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: At the day of the treatment the animals were controlled several times. During the 14-day observation period they were monitored twice per day ( once per day over weekends and holidays). The body weights were recorded at the day of the treatment, after 1 week and the end of the observation period.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, gross pathology

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 5 000 mg/kg bw
Based on:
test mat.
Mortality:
No mortality was observed in the male and female group dosed with 3100  mg/kg bw. 2/5 males and 2/5 females dosed with 5000 mg/kg bw died within the first  week post application.
Clinical signs:
bodyweight loss
Body weight:
other body weight observations
Gross pathology:
Gross autopsy of some of the surviving rats showed no pathological  findings.

Any other information on results incl. tables


Table 1.Effect of acute oral toxicity test


 


























































Doses



Toxicological results



Time of death



Start of the symptoms



mL/kg



Dead animals



Animals with symptoms



Total of animal employed



 



 



Males



3.1



0



3



5



 



7 d



5.0



2



5



5



2-3d



24 h



Females



3.1



0



4



5



 



 



5.0



2



5



5



2-6 d



24 h



 


Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Combined LD50 > 5000 mg/kg bw/day.
Executive summary:

Löser E (1982)


 


In order to test the acute oral toxicity of 1,2,3 -trichlorobenzene, 5 male and 5 female tested were dosed by gavage (3100 and 5000 mg/kg bw) with a method similar to OECD guideline 401 with deviations (No analytical purity reported. No GLP. Only 2 doses tested)


 


The following toxicological effects were observed:


1) Mortality: no mortality was observed in the male and female group dosed with 3100  mg/kg bw. 2/5 males and 2/5 females dosed with 5000 mg/kg bw died within the first  week post application.


 


2) Signs of intoxication
Rats of both sex and in both dose groups suffered from weight loss, rough  fur, bloody snout and reduced general condition.


 


3) Gross autopsy 
Gross autopsy of some of the surviving rats showed no pathological  findings.