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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Method similar to OECD guideline with restrictions

Data source

Reference
Reference Type:
publication
Title:
Toxicitiy of selected chlorobenzenes to aquatic organisms
Author:
Calamari, D., Galassi, S., Setti, F., Vighi, M.
Year:
1983
Bibliographic source:
Chemosphere 12, 253-262

Materials and methods

Principles of method if other than guideline:
Daphnia magna (ca. 12 hours old) were exposed to different concentrations of 1,2,3-trichlorobenzene for about 14 days until at least three broods were obtained. The animals in groups of 10 were kept in 500 mL closed bottles, completely filled with IRCHA standard water added with the test material, 8 mg/L dried wtight Selenastrum capricornutum and 200 mg/L wet weight commercial yeast. The bottles (3 replicates per test group) were kept in a climatic room at 20 °C with a 16 h light photoperiod. The solutions were changed every other day, at the same time the numbers of dead and newborn animals were observed and removed. Samples of water were taken for the gas-chromatographic analysis of the test concentration after 48 hours. During the test, the loss of compound did not exceed 15% of the initial value. Repeated measurements of pH and O2 in the test solutions did not show fluctuation higher than 10%. Fertility data were elaborated according to Winner and Farrel (1976) to obtain instantaneous growth rates, while multiple controntations in the variance analysis were adopted to find the concentrations which gave differences of r significant compared with the control.
Winner RW and Farrel MP (1976): J. Fish. Res. Board Canada, 33: 1685-1691
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
other: EC16
Effect conc.:
0.08 mg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
reproduction
Remarks on result:
other: The EC16 is viewed as being the NOEC
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
0.2 mg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
reproduction
Duration:
14 d
Dose descriptor:
LOEC
Effect conc.:
0.1
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
reproduction
Details on results:
The EC16 is reported as no effect concentration due to the accepted hypothesis that a reproductive impairment ranging from 10 to 20% could be considered in the range of natural variability.

Applicant's summary and conclusion