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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge nitrification inhibition testing
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Reference Type:
publication
Title:
A database of chemical toxicity to environmental bacteria and ist use in interspecies comparisons and correlations
Author:
Blum DJW and RE Speece
Year:
1991
Bibliographic source:
Research Journal WPCF 63(3): 198-207

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
ISO 9509 (Toxicity test for assessing the inhibition of nitrification of activated sludge microorganisms)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1,2,3-trichlorobenzene
EC Number:
201-757-1
EC Name:
1,2,3-trichlorobenzene
Cas Number:
87-61-6
Molecular formula:
C6H3Cl3
IUPAC Name:
1,2,3-trichlorobenzene

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Nitrosomonas sp.

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Test conditions

Test temperature:
25°C
pH:
6.5-8.0
Details on test conditions:
- Test vessel: 125 mL serum bottle
- Liquid Volume: 50 mL
- Shaking: yes

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
IC50
Effect conc.:
96 mg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
other: Ammonia use

Applicant's summary and conclusion