Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 231-743-0
CAS number: 7718-54-9
Mouse Ear Swelling Test Results: The intradermal application with
a nickel chloride solution produced cutaneous inflammation in mice,
although these reactions were not significantly different from those
obtained in mock-sensitised mice.
Effect of Sensitization Regimen on MEST and LLNA results: The
ear-swelling 48 h after the challenge did not differ between sensitised
and not sensitised mice, and the adjuvants and the excipients did not
affect the ear-swelling and erythema when compared to mice sensitised
with Ni(lII) alone; neither were the CD4-positive T-cell counts affected
by sensitization regimen (Table 1 below). In addition, in vitro
proliferation of auricular lymphocytes did not suggest that
sensitisation with nickel induced a specific CD4 T-cell responses.
Table 1. Erythema, ear-swelling, and lymphocyte activation in mice
sensitised by intradermal injections of nickel chloride.
Readout and challenge regime
a - Mice were individually scored with 0 (no erythema), 1 (notable
erythema), or 2 strong erythema and an average was calculated.
b - The average MEST (swelling of ear compared 10 baseline) Is indicated
c - Number of CD4-positive lymphocytes in auricular lymph nodes was
calculated based on the number of lymph node cells (counted in
hemocytometer) and the percentage of CD4 T-cell subsets (analysed by
flow cytometry). No statistical differences were detected using the
Kruskal-Wallis non-parametric H-test with Dunn's multiple comparison
d - Stimulation index of lymph cells re-stimulated in vitro with 100 uM
Ni(II) in the presence of [3H]-thymidine for 16 h. No statistical
differences were detected using the Kruskal-Wallis non-parametric H-test
with Dunn's multiple comparison test (n=5).
*Ni(III) was made by mixing NiCl2 with a 20 -fold molar excess of H2O2.
Effects of titrating NiCl2 amounts in sensitized and non-sensitized
mice: There was a dose-dependent swelling of the ear as NiCl2 levels
were increased, but no difference was found between sensitized and
non-sensitised mice (p> 0.05 by two-way ANOVA). The cellular infiltrate
in the swollen ears was identical in sensitised and non-sensitised mice
as measured by histology.
Effects of NiCl2 in lymphocyte deficient mice: The exclusion of
any lymphocytic involvement in the skin reactions was further supported
by similar experiments performed in lymphocyte deficient mice. The
cutaneous inflammation observed in T-cell receptor knock-out mice and in
athymic nude mice did not differ from that observed in wild type mice.
MEST results in mice bred in Ni-free environment: There was no
difference in the ear-swelling reactions in mice bred under conventional
conditions and in mice bred and kept under nickel-free conditions.
Moreover, for mice kept under nickel-free conditions, the skin reactions
following a challenge with Ni(II) chloride were similar in
nickel-sensitised and in nonsensitised mice.
Study rated by an independent reviewer.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
På den här webbplatsen används kakor. Syftet är att optimera din upplevelse av den.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again