3a,4,7,7a-tetrahydro-4,7-methanoindene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 September 1988 - 6 October 1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

GLP compliant, guideline study, available as unpublished report, no restrictions, fully adequate for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

Principles of method if other than guideline:

n/a

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reference substance 001

Cas Number:

77-73-6

Molecular formula:

C10H12

Test material form:

liquid

Details on test material:

- Supplier: Dow Chemical Europe SA
- Batch number (Sponsor's identification): DCPD 75%
- Name of test material (as cited in study report): DCPD 75%
- Physical state: clear, yellow-coloured liquid
- Composition of test material, percentage of components: 71.1% endo dicyclopentadiene, 0.8% exo dicyclopentadiene, 1.4% m-bicyclozonadiene, 15.2% CPD-MCPD codimers, 0.3% tricyclopentadiene, 1.3% CPD-butadiene codimer, 0.3% CPD-piperylene codimer, 0.3% CPD-isoprene codimer, <0.1% benzene , remainder misc. hydrocarbons.
- Specific gravity: 0.971
- Storage condition of test material: room temperature

Specific details on test material used for the study:

- Supplier: Dow Chemical Europe SA
- Batch number (Sponsor's identification): DCPD 75%- Name of test material (as cited in study report): DCPD 75%
- Physical state: clear, yellow-coloured liquid
- Composition of test material, percentage of components: 71.1% endo dicyclopentadiene, 0.8% exo dicyclopentadiene, 1.4% m-bicyclozonadiene, 15.2% CPD-MCPD codimers, 0.3% tricyclopentadiene, 1.3% CPD-butadiene codimer, 0.3% CPD-piperylene codimer, 0.3% CPD-isoprene codimer, <0.1% benzene , remainder misc. hydrocarbons.
- Specific gravity: 0.971
- Storage condition of test material: room temperature

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Interfauna (UK) Ltd., Wyton, Huntingdon, Cambridgeshire, UK
- Age at study initiation: 8-12 weeks
- Weight at study initiation: males 231-256 g; females 210-255 g
- Fasting period before study: None
- Housing: Solid floor polypropylene cages with sawdust bedding
- Diet: Rat and Mouse expanded Diet No. 1 (Special Diet Services Ltd., Witham, Essex, UK) ad libitum
- Water: Mains drinking water ad libitum
- Acclimation period: At least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature: 20-21°C
- Humidity: 45-68%
- Air changes: approximately 15 per hour
- Photoperiod: 12 hrs dark / 12 hrs light

IN-LIFE DATES: From: 22 September 1988 To: 6 October 1988

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: shorn skin on back and flanks
- % coverage: 10%
- Type of wrap if used: aluminium foil occluded with double layers of adhesive strapping wound around trunk of animal

REMOVAL OF TEST SUBSTANCE
- Washing (if done): with moist cotton wool
- Time after start of exposure: 24 hours

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2.06 mL/kg bodyweight
- Constant volume or concentration used: yes

Duration of exposure:

24 hours

Doses:

2000 mg/kg bodyweight

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observed 1 and 4 hours after dosing and daily thereafter for 14 days. Bodyweights recorded on day of treatment and on days 7 and 14
- Necropsy of survivors performed: no

Statistics:

None, acute LD50 estimated.

Results and discussion

Preliminary study:

n/a

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

None

Clinical signs:

other: other: Vocalisation, lasting up to 30 minutes, noted in all animals after dosing. Hunched posture, lethargy, piloerection, erythema and oedema present in all animals on day 1. Continued in 'Any other information on results'

Body weight:

other body weight observations

Gross pathology:

No abnormalities were seen.

Other findings:

n/a

Any other information on results incl. tables

Clinical signs continued:

Isolated incidences of red/brown staining of snout and ptosis observed. These reactions regressed and by Day 3 all animals showed signs of eschar which persisted until Day 10 and Day 12 in two animals. All treatment sites appeared normal by the end of the study period.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Remarks:

Criteria used for interpretation of results: EU

Conclusions:

The acute dermal LD50 of dicyclopentadiene 75% to the rat was greater than 2000 mg/kg body weight.

Executive summary:

The acute dermal toxicity of dicyclopentadiene 75% was assessed in a group of 5 male and 5 female rats. 2.06 mL/kg body weight was applied to the shorn flank and held in place with an occlusive dressing. Animals were observed at 1 and 4 hours after dosing and then daily for 14 days. Clinical signs present on day 1 included vocalisation lasting up to 30 minutes (noted in all animals after dosing), hunched posture, lethargy, piloerection, erythema and oedema, . Isolated incidences of red/brown staining of snout and ptosis were seen. All animals showed signs of eschar by day 3 which persisted until days 10 or 12. All treatment sites appeared normal by the end of study. All animals gained weight and there were no gross abnormalities at necropsy. The acute dermal LD50 of dicyclopentadiene 75% in the rat was greater than 2000 mg/kg body weight.