3a,4,7,7a-tetrahydro-4,7-methanoindene

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Final report, July 1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, similiar to standard guideline, published, minor restrictions in design and/or reporting but otherwise adequate for assessment

Qualifier:

equivalent or similar to guideline

Guideline:

other: Macroinvertebrate and fish toxicity tests followed the recommended bioassay procedures as described in the “Methods for Acute Toxicity Tests with Fish, Macro invertebrates, and Amphibians” (US EPA 1975)

Deviations:

not specified

Principles of method if other than guideline:

Macroinvertebrate and fish toxicity tests followed the recommended bioassay procedures as described in the “Methods for Acute Toxicity Tests with Fish, Macro invertebrates, and Amphibians” (US EPA 1975)

GLP compliance:

no

Specific details on test material used for the study:

Dicyclopentadiene (DCPD): clear liquid received from Aldrich Chemical company, Milwaukee, Wisconsin. The DCPD was tested on a 95% active ingredient basis.

Analytical monitoring:

yes

Details on sampling:

Dissolved oxygen contration, pH and temperature of test solutions were checked at 0, 48 and 96 hrs in 2 selected test concentrations at a minimum.

Vehicle:

yes

Remarks:

acetone was used as a solvent/carrier

Details on test solutions:

Stock solutions were prepared in a solution of reagent-grade acetone. Stock solution for fish ration of 1.5 parts DCPD : 98.5 parts acetone (volume:volume). Negative controls, consisting of the same dilution water and conditions as test concentrations but no DCPD

Test organisms (species):

other: Ictalurus punctatus

Details on test organisms:

Mean wet weight of channel catfish was 1.3 g.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

N/A

Post exposure observation period:

not reported

Hardness:

not reported

Test temperature:

21 ± 1.0 °C for channel catfish.

pH:

6.9 - 7.3

Dissolved oxygen:

3.8 - 8.8 mg/L

Salinity:

not reported

Conductivity:

N/A

Nominal and measured concentrations:

nominal concentrations (mg/L): control, control (acetone), 14.0, 16.0, 18.0, 24.0, 32.0

Details on test conditions:

Static fish bioassays were conducted in 19.6 L glass vessels held in contact temperature water baths at 21±1.0.°C for the channel catfish. The standard diluents (well water) used had the same water quality characteristics as that for holding water.

Reference substance (positive control):

yes

Remarks:

control (acetone)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

15.7 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

not specified

Details on results:

Reported as median lethal concentration

Results with reference substance (positive control):

Results showed in the table included in section "Any other information on results inc. table" show no mortality in the control (acetone) group

Reported statistics and error estimates:

95% confidence levels

Sublethal observations / clinical signs:

Table: Concentrations tested and corresponding observed percentage mortalities for channel catfish (Ictalurus punctatus) exposed to dicyclopentadiene ( DCPD) for 24 , 48 and 96 hours .

Species	Nominal concentration (mg/L)	% motality observed
		24 h	48 h	96 h
	Control	0	0	0
	Control (acetone)	0	0	0
	14.0	0	0	0
	16.0	0	40	60
	18.0	20	20	100
	24.0	60	60	100
	32.0	100	100	100

Validity criteria fulfilled:

not applicable

Remarks:

This is a non standard study

Conclusions:

This is a 96 h LC50 experimental study, non GLP and similar to standard guidelines, with notable restrictions in design and/or reporting but contributing to weight of evidence assessment. The 96 h LC50 Ictalurus punctatus was reported to be 15.7 mg/L.

Executive summary:

This is a 96 h LC₅₀ experimental study, non-GLP and similar to standard guidelines, with notable restrictions in design and/or reporting but contributing to weight of evidence assessment. The 96 h LC₅₀ Ictalurus punctatus was reported to be 15.7 mg/L

Description of key information

The 96 hour LC₅₀ is 15.7 mg/L. The estimated LC₅₀ value from TLM QSAR is 6.712 mg/L.

The experimental value was used for the chemical safety assessment.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Remarks:

96 hour LC50

Effect concentration:

15.7 mg/L

Additional information

An Environmental Agency Japan test following OECD guideline 203, using semi-static conditions without analytical confirmation of exposure concentrations showed the 96-hour LC₅₀ to be 4.3 mg/L. This study was unavailable for review but was included in the OECD SIDS for DCPD.

Bentley et al (1976) tested the toxicity of dicyclopentadiene to four species of fish. The range of LC50 values reported within the paper was between 15.7 -31.1 mg/L. The study followed a method similar to standard guidelines but did not include analytical confirmation of the exposure concentrations.

A QSAR result has also been included as part of the weight of evidence, which used the ECOSAR program to derive a 96-hour LC₅₀ of 9.8 mg/L and Target Lipid modelling to derive an estimated LL₅₀ for this substance is 6.712 mg/L.

The lowest of the measured results which are considered to be reliable has been conservatively selected as the key value for this endpoint.

The 96-hour LC₅₀ for freshwater fish is 15.7 mg/L (Bentley et al 1976).