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The effect of DCPD on the nitrification of soil microorganisms was determined in a GLP-compliant study following OECD guideline 216. No inhibiting effect on the nitrification potential was observed up to a concentration of 1000 mg/kg SDW, the highest concentration tested. The NOEC is therefore considered to be =1000 mg/kg SDW.

The effect of DCPD on earthworm reproduction was determined in a GLP-compliant study following OECD guideline 222. Earthworms were exposed to 6 concentrations of DCPD alongside a control in artifical soil. There were no statistically significant differences in earthworm reproduction in the treatment rates 15.6 to 125 mg DCPD/kg SDW compared to the control. However, at the test item concentration 250 mg DCPD/kg, the earthworm reproduction was statistically significantly reduced and, at the test item concentration 500 mg DCPD/kg soil dry weight, no juveniles were found. Overall, the NOEC of the test item concerning mortality, biomass and reproduction was determined to be 125 mg DCPD/kg SDW and based on reproduction. The EC50-value for reproduction was determined to be 250 mgDCPD/kg SDW.

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