Methyl formate

Toxicity to reproduction

Administrative data

Endpoint:

fertility, other

Remarks:

based on test type (migrated information)

Type of information:

other: Experimental study with methanol, a metabolite of methyl formate.

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedures based on scientific principles and standards, limited documentation, acceptable for assessment.

Data source

Reference

Reference Type:

publication

Title:

Sperm count, morphology, and fluorescent body frequency in autopsy service workers exposed to formaldehyde.

Author:

Ward, J. B. et al.

Year:

1984

Bibliographic source:

Mutat Res 130: 417-424

Materials and methods

Principles of method if other than guideline:

Male mice were treated orally with methanol and maintained without exposure for 5 weeks. Sperm morphology was examined.

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methanol

EC Number:

200-659-6

EC Name:

Methanol

Cas Number:

67-56-1

Molecular formula:

CH4O

IUPAC Name:

Methyl alcohol

Details on test material:

- Name of test material (as cited in study report): methanol
- Analytical purity: no data

Test animals

Species:

mouse

Strain:

B6C3F1

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Breeding Laboratory, Portage, MI
- Age at study initiation: 4-month old

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on exposure:

no data

Details on mating procedure:

not applicable

Analytical verification of doses or concentrations:

not specified

Details on analytical verification of doses or concentrations:

no data

Duration of treatment / exposure:

5 consecutive days

Frequency of treatment:

daily

Doses / concentrations

Remarks:

Doses / Concentrations:
1000 mg/kg bw
Basis:
nominal conc.

No. of animals per sex per dose:

10

Control animals:

yes, concurrent vehicle

Examinations

Statistics:

The effects of exposure and non-exposure for each factor were compared by Student´s t-test.
Statistical analyses were performed using the Statistical Package for the Social Sciences (SPSS) [Nie et al., 1975].

Results and discussion

Results: P0 (first parental generation)

Effect levels (P0)

Dose descriptor:

NOAEL

Effect level:

< 1 000 other: mg/kg

Sex:

male

Basis for effect level:

other: sperm morphology

Overall reproductive toxicity

Reproductive effects observed:

not specified

Any other information on results incl. tables

In the methanol-treated animals, a slight, but statistically insignificant increase in sperms abnormalities (1.86 +-0.91% vs. 1.12 +-0.39% in the water control) was observed, while the treatment with cyclophoshamide (100 mg/kg) resulted in an about 5-fold increase (5.84 +-1.94).