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Toxicological information

Neurotoxicity

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Administrative data

Endpoint:
neurotoxicity
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards, well documented and acceptable for assessment. Restrictions: purity of test substance not reported. Tested dose levels not reported.

Data source

Reference
Reference Type:
publication
Title:
Aliphatic alcohols and alkyl esters: narcotic and lethal potencies to tadpoles and rabbits.
Author:
Munch JC
Year:
1972
Bibliographic source:
Industrial Medicine 41, 31-33.

Materials and methods

GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
TS-Freetext:
Homologous series of aliphatic alcohols and their alkyl
esters. Included methyl formate.

Test animals

Species:
rabbit
Sex:
male/female

Administration / exposure

Route of administration:
other: oral
Vehicle:
physiological saline
Frequency of treatment:
Single dose
Details on study design:
Observation period: 24 hours

Results and discussion

Any other information on results incl. tables

RS-Freetext:
(1) Narcotic and lethal doses of methyl formate:
ND50 = 27 millimols/kg bw.
LD50 = 27 millimols/kg bw.
                  
27 millimols/kg bw correspond to 1620 mg/kg bw.

(2) Margin of safety: LD50/ND50=1.0

(3) The narcotic potential and the toxicity of the methyl
esters (formate, acetate, propionate, butyrate, isovalerate)
were much higher (by factors of 5-10) than the alcohol,
methanol. To a lower extent this effect was also seen with
ethanol, whereas in the C3-C5 series the potencies of the
alcohol and their esters were comparable to each other.

The narcotic potential and toxicity of the methyl esters
(only formate shown in the table below) was much higher
compared to the alcohol.

====================================================
                  ND50         LD50    [mmol/kg bw]         
----------------------------------------------------
Methanol:        187.5         450
methyl formate:   27            27   
====================================================

Applicant's summary and conclusion

Conclusions:
In rabbits, narcosis (ND50) and mortality (LD50) occurred
with methyl formate at the same oral dose (approx. 1620
mg/kg bw). Thus there was no margin of safety.

The narcotic potential and toxicity of the ester was much
higher compared to the alcohol.