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EC number: 203-481-7 | CAS number: 107-31-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl formate
- EC Number:
- 203-481-7
- EC Name:
- Methyl formate
- Cas Number:
- 107-31-3
- Molecular formula:
- C₂H₄O₂
- IUPAC Name:
- methyl formate
- Details on test material:
- - Test substance: Methyl formate
- Analytical purity: 98.4 %.
Constituent 1
Method
- Target gene:
- operon coding for histidine biosynthesis
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- from Aroclor 1254 induced rat liver
- Test concentrations with justification for top dose:
- 20 - 5000 µg/plate
- Vehicle / solvent:
- - Vehicle used: DMSO
- Justification for choice of solvent/vehicle: commonly used
Controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- no
- Remarks:
- Migrated to IUCLID6: Without S-9 mix: N-methyl-N'-nitro-N-nitroso-guanidine, 4-nitro-o-phenylenediamine, 9-aminoacridine. With S-9 mix: 2-aminoanthracene.
- Details on test system and experimental conditions:
- - Standard plate test, SPT: according to Ames.
- Preincubation assay, PIA: 20 minutes at 37°C, according to Yahagi et al. (1977) and Matsushima et al. (1980).
- Metabolic activation: with and without metabolic microsomal enzyme systems (S-9 fraction) from Arochlor 1254-induced male Sprague-Dawley rat liver were used.
- Concentrations:
- 1st experiment (SPT, w/wo metabolic activation; TA1535, TA 100, TA1537, TA98): 0, 20, 100, 500, 2500, and 5000 µg/plate.
- 2nd experiment (SPT, w/wo metabolic activation; TA1535): 0, 100, 500, 750, and 1000 µg/plate.
- 3rd experiment (PIA, w/wo metabolic activation; TA1535, TA 100, TA1537, TA98): 0, 20, 100, 500, 2500, and 5000 µg/plate.
- Replicates: 3 plates per dose or control in each of the experiments. - Evaluation criteria:
- Requirements to characterize a substance as positive:
- doubling of the spontaneous mutation rate
- dose-response relationship
- reproducibility.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- > 5000 µg/plate
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
-
ADDITIONAL INFORMATION ON CYTOTOXICITY:
No bacteriotoxicity was noted at any dose including the highest tested dose of 5000 µg/plate. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Methyl formate did not increase the number of revertants in any of the experiments, with or without metabolic
activation.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative - Executive summary:
Conclusion:
Methylformate (20 – 5000 µg/plate) was negative in a valid Ames test conducted according to OECD 471 using S. typhimurium strains TA1535, TA 1537,TA98, and TA100, with and without metabolic activation. Positive and negative controls performed as expected.
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