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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions. Restriction: not reported whether treatment-related body weight changes observed.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report Date:
1978

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
yes
Remarks:
; body weight was not controlled
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Test substance: Methyl formate
- Analytical purity: 9
- Impurity. methanol 2%.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: males 250 g, females 180 g

Administration / exposure

Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
TEST SITE
- Area of exposure: backside, 42 cm²
- % coverage:

Doses:
2000 and 4000 mg/kg bw
No. of animals per sex per dose:
3
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: animals were observed daily for signs of toxicity. Mortalities were recorded.
- Necropsy of survivors performed: yes/no all animals were examined after death or at termination.
- Other examinations performed: clinical signs, body weight,organ weights, histopathology.

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 4 000 mL/kg bw
Mortality:
No mortality seen at any dose within the 14-d observation period.
Clinical signs:
Slight apathy, stagger, unsteady gait, unregular breathing. No signs of local irritation noted.

Applicant's summary and conclusion

Executive summary:

Conclusion:

There were no deaths (0/12) in rats after dermal contact to methyl formate at the Limit Test concentration and at 4000 mg/kg bw.