Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions (no analytical verification of test item concentrations; non-GLP)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Methyl formate
- Analytical purity: 97%

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna Straus
- Age at study initiation (mean and range, SD): 2 - 24 h


Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.70 mmol/L
Test temperature:
19 - 21 °C
pH:
7.7 - 8.3
Dissolved oxygen:
Oxygen content was within acceptable limits throughout the test.
Nominal and measured concentrations:
Nominal: 0, 62.5, 125, 250, and 500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): test tubes (glass) with flat bottom (nominal volume: 20  ml)
- Material, size, headspace, fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- total number of animals/concentration: 20


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water with the following pretreatment 
(1) 6 µm- and charcoal-filtration
(2) H2SO4 was added to reduce alkalinity up to pH 4.3
(3) distilled water was added to reduce water-hardness
(4) water was aerated (oil-free air) until saturated with oxygen
(5) water was stored for at least 24 h for stabilization
- Alkalinity up to pH 4.3: 0.80 mmol/l
- Conductivity: 550 -  640 µSiemens/cm)  


OTHER TEST CONDITIONS
- illumination: diffuse light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- observation times: visually after 0, 3, 6, 24 and 48 h
- observation parameters: swimming ability, pH, oxygen 

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility

Any other information on results incl. tables

There was no mortality in any concentration tested at any time point  thoughout the test.

Temperature, pH, and oxygen content were within acceptable limits throughout the test.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes