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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions (no analytical verification of test item concentrations; non-GLP)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: German Industrial Standard DIN 38 412, Part 15
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Methyl formate
- Analytical purity: 97.7%

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Leuciscus idus L., golden variety (golden orfe)
- Length at study initiation (length definition, mean, range and SD): 6.9 cm (range: 6.3 - 7.5 cm)
- Weight at study initiation (mean and range, SD): 2.7 g (range: 2.0 - 3.6 g)
- corpulence factor of the batch: 0.8


ACCLIMATION
- Acclimation period: 3 days
- Acclimation conditions (same as test or not): same as in test
- Feeding frequency: withdrawal of food 1 day before and during exposure

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
2.5 mmol/L
Test temperature:
21 °C
pH:
6.6 - 7.6
Dissolved oxygen:
Oxygen levels remained within normal ranges throughout the study
Nominal and measured concentrations:
Nominal: 0.0 (control) 10.0, 21.5, 46.4, 100.0, and  215.0 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): non-sealed all-glass aquaria (30x22x24 cm)
- Material, size, headspace, fill volume: 10 L
- Aeration: slight, continuous with oil-free air
- No. of organisms per concentration: 10
- Biomass loading rate: 2.7 (g fish / L test water)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater was prepared from fully demineralized tap water according to DIN 38 412, part 11 (1 982) that was resalted by the addition of 294.0 mg/l CaCl2*2H2O, 123.3 mg/l MgSO4*7H2O, 63 mg/l NaHCO3 and 5.5 mg/l KCl
- Ca/mg ratio:4:1


OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 h darkness


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality after 1, 4, 24, 48, 72, and 96 h; measurements: temperature, pH, and oxygen content after 1, 24, 48, 72,  and 96 h


Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
46 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
46 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 115 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
215 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality

Any other information on results incl. tables

Mortality:

Nominal concentration (mg/L)

Cumulative mortality (n)

 

1 h

4 h

24 h

48 h

72 h

96 h

0 (control)

0

0

0

0

0

0

10.0

0

0

0

0

0

0

21.5

0

0

0

0

0

0

46.4

0

0

0

0

0

0

100.0

0

0

1

3

3

3

215.0

0

0

10

10

10

10


From these data, an LC50 of ca. 115 mg/l was derived by interpolation.

Adverse clinical signs were limited to "tumbling" for the 100 mg/l group at the 24 hour observation and the 215 mg/l
group at the 4 hour observation.

Oxygen levels and pH remained within normal ranges throughout the study. The recorded temperature remained at 21° C at all measurements.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes