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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
no
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl formate
EC Number:
203-481-7
EC Name:
Methyl formate
Cas Number:
107-31-3
Molecular formula:
C₂H₄O₂
IUPAC Name:
methyl formate
Details on test material:
- Name of test material (as cited in study report): Methylformiat
- Physical state: liquid
- Analytical purity: 98%
- Impurities (identity and concentrations): methanol 2%.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 190-270 G (males) and 180-210 g (females).
- Fasting period before study: 15-20 h 
- Diet: Herilan MRH-Haltung, Eggersmann KG

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 4.64 - 21.5%

MAXIMUM DOSE VOLUME APPLIED: 10 ml/kg bw.
Doses:
464, 681, 1000, 1470, 2150 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: after administration, at least daily. Weighing: befor administration and on day 2-4, 7 and 13
- Necropsy of survivors performed: yes
- Other examinations performed: gross pathology of all animals that died

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 1 500 mg/kg bw
Mortality:
Deaths occurred within 1 hour after dosing at the two highest dose levels (2150 mg/kg bw: 10/10 animals; 1470 mg/kg bw: 4/10).
No deaths occurred in the other dose groups.
Clinical signs:
464 and 681 mg/kg bw:  None;
1000 mg/kg bw:  Dyspnea, apathy, poor condition.
1470 mg/kg bw:  Dyspnea, apathy, stagger, unsteady gait, poor  condition.
2150 mg/kg bw:   Dyspnea, apathy, stagger, unsteady gait, unkempt fur, cyanosis, poor condition, gasps.
Gross pathology:
Animals that died: 
Lungs: rich in blood, edema. Glandular stomach: caustic gastritis. Intestine: softening of the mucosa due to caustic corrosion.
No findings in sacrificed rats.

Any other information on results incl. tables

Symptoms
==========================================================
Dose groups     Symptoms
(mg/kg bw)
----------------------------------------------------------
 464          None
 681          None
1000          Dyspnea, apathy, poor condition.
1470          Dyspnea, apathy, stagger, unsteady gait, poor 
             condition. 
2150          Dyspnea, apathy, stagger, unsteady gait,      
             unkempt fur, cyanosis, poor condition, gasps. 
==========================================================  

Applicant's summary and conclusion

Executive summary:

Conclusion:

The acute oral toxicity of methylformate was examined in a pre-GLP gavage study that was conducted similar to OECD Guideline No.401 using 5 Sprague-Dawley rats per sex per dose. The dose levels tested were 464, 681, 1000, 1470, 2150 mg/kg bw. The observation period was14 days. The combined LD50was approx. 1500 mg/kg bw in this study.