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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study. According to OECD TG 403 no full study with three dose levels required because there were no mortalities in the Limit Test at a 5.2 mg/l.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl formate
EC Number:
203-481-7
EC Name:
Methyl formate
Cas Number:
107-31-3
Molecular formula:
C₂H₄O₂
IUPAC Name:
methyl formate
Details on test material:
- Test substance: Methylformiat
- Analytical purity: 98 %

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source:  MUS RATTUS, Brunnthal.
- Weight at study initiation:  males: 187 g, females: 188 g.
- Fasting period before study:
- Housing:  in groups of 5 animals in wire mesh cages
- Diet: ad libitum, Herilan MRH, H. Eggersmann KG, Rinteln, Germany
- Water: ad libitum
- Acclimation period:

ENVIRONMENTAL CONDITIONS
- Temperature (°C):
- Humidity (%):
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light):

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Details on inhalation exposure:
- Exposure System: whole-body exposure. 
- Inhalation exposure chamber: glass-steel construction, volume 200 litres.
- Exposure: cages with 5 animals each were placed into the inhalation chamber.
- Duration of exposure period: 4 h. 
- Atmosphere generation: continuous delivery of test substance using an infusion pump to an all-glass evaporator heated to 75°C.
Analytical verification of test atmosphere concentrations:
yes
Remarks:
Analytical method: gas chromatography, following sampling in 2-propanol.
Duration of exposure:
4 h
Concentrations:
5.19 mg/l
No. of animals per sex per dose:
20
Control animals:
yes
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: on days 0, 7, and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:
Statistics:
Statistical evaluation: using the binominal test described by Wittig (1974).

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 5.2 mg/L air
Exp. duration:
4 h
Mortality:
No mortalities in the male (0/10) or female (0/10) group within the 14-d observation period.
Clinical signs:
other: Discharge of the conjunctivae during exposure, unkempt fur thereafter, forced breathing. No symptoms were seen the second day following exposure.
Body weight:
There was no difference between male and female treated and control groups regarding body weight and body weight gain at 7 and 14 days post treatment.
Gross pathology:
No findings in sacrificed animals.

Any other information on results incl. tables

Mean analytical concentration of TS: 5.19 mg/l.

Applicant's summary and conclusion