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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
other: read-across to hydrolysis product (formate ion)
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Formic acid
- Analytical purity: 99.4 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna Straus
- Age of parental stock (mean and range, SD): 2-4 weeks
- Age of test animals: 2- < 24 h
- Feeding during test
- Food type: algal suspension (Desmosdesmus subspicatus)
- Amount: 0.10-0.20 mg TOC/parent animal/day
- Frequency: daily

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d

Test conditions

Test temperature:
19.5-20.4 °C
pH:
7.0-8.3
Dissolved oxygen:
8.0-9.5 mg/l
Nominal and measured concentrations:
0.0, 1.0, 3.2, 10, 32 (neutralised), and 100 (neutralised) mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100-ml glass beakers, covered with glass caps
- Material, size, headspace, fill volume: 50 mL
- Renewal rate of test solution (frequency/flow rate): semistatic conditions: renewal on Mondays, Wednesdays, Fridays
- No. of vessels per concentration (replicates): 10
- Biomass loading rate: 1 parent animal/vessel, 50 mL/parent animal

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater according to OECD guideline 211

OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h darkness
- Light intensity: artificial light, type warm white (OSRAM L58 W31)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- measurements:       
- number of young daphnids (daily)      
- mortality, embryos, aborted eggs (daily)      
- weight and length of parent animals (end of exposure)      
- test substance (at renewal of test solution: old and new test solution)      
- pH and dissolved oxygen (at renewal of test solution: old and new test solution)      
- temperature (continuously in separate vessel)

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
other: EC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
immobilisation
Reported statistics and error estimates:
For the statistical evaluation of the LOEC and NOEC Dunnett's test was used to analyse the parameters: 
- number of living young (one-sided analysis)
- length and weight (two-sided analysis).
- Dunnett CW (1955). J Ameri Statist Assoc 50: 1096-1121.
- Dunnett CW (1964). Biometrics 20: 482-491.

Any other information on results incl. tables

No adverse effects (reproduction, weight, length) were observed up to the highest concentration of 100 mg/l. 

NOEC >= 100 mg/l
LOEC > 100 mg/l

swimming ability of parent animals:
EC0 >= 100 mg/l


Nominal conc. (mg/L)

Total number of living young per surviving parent animal after 21 d exposure

 

min

max

mean

sd

cv (%)

0

91

150

109.7

15.8

14.4

1

88

113

102.6

7.5

7.33

3.2 *

94

135

110.2

14.8

13.4

10

79

141

108.7

19.0

17.5

32 n

85

137

105.5

14.6

13.8

100 n

92

129

110.0

11.9

10.8

*: 1 parent animal died on day 16
n: neutralised

Nominal conc. (mg/L)

Parental animal condition after 21 d exposure

 

weight (mg)

length (mm)

 

mean

sd

cv (%)

mean

sd

cv (%)

0

1.0

0.2

17.64

3.98

0.17

4.23

1

1.0

0.3

24.83

3.93

0.10

2.51

3.2 (*)

1.1

0.2

15.64

4.05

0.17

4.30

10

1.1

0.2

18.19

3.97

0.11

2.66

32n

1.0

0.1

13.79

3.91

0.09

2.42

100n

1.0

0.2

21.21

3.95

0.11

2.87

*: 1 parent animal died on day 16
n: neutralised


- test substance concentrations: The analytically determined concentrations of the test substance were in a range of +- 20 %

 of the nominal concentration in all tested concentrations and remained constant over the exposure period.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes