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Ecotoxicological information

Additional ecotoxological information

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Administrative data

Endpoint:
additional ecotoxicological information
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2016
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Thyroid hormone-disrupting activity and ecological risk assessment of phosphorus-containing flame retardants by in vitro, in vivo and in silico approaches
Author:
Zhang Q, Ji C, Yin X, Yan L, Lu M, Zhao M
Year:
2016
Bibliographic source:
Environ Pollut, 210, 27-33

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Triphenyl phosphate
EC Number:
204-112-2
EC Name:
Triphenyl phosphate
Cas Number:
115-86-6
Molecular formula:
C18H15O4P
IUPAC Name:
triphenyl phosphate

Results and discussion

Any other information on results incl. tables

No agonistic or antagonistic activity of Triphenyl phosphate against the TRß receptor was observed. While the publication reported results with CHO-K1 cells and GH3 cells only those with the CHO-K1 cells are reported here as this is a hamster cell line, which is considered relevant in this IUCLID chapter. GH3 cells are rat pituitary adenoma cell lines instead.

Applicant's summary and conclusion

Conclusions:
No agonistic or antagonistic activity of Triphenyl phosphate against the TRß receptor was observed.
Executive summary:

Potential interaction of TPP with TRß receptor in CHO-K1 cells were assessed in an non-standard in vitro test system with the Dual-Luciferase® Reporter Assay System purchased from Promega (Madison, WI, USA). 0.1 % DMSO was used as a solvent. The test system is static and the bioassays lasts 24 hours. T3 hormone was used as a positive control. The bioassay allows to assess interactions which are agonistic or antagonistic against TRß receptor.

No agonistic or antagonistic activity of Triphenyl phosphate against the TRß receptor was observed. Thus, TPP showed no potential to interact with