Triphenyl phosphate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study conducted according to national standard methods without detailed documentation (pH, dissolved oxygen and water hardness of test medium not stated) - (also assessed by OECD)

Qualifier:

according to guideline

Guideline:

other: see below

Principles of method if other than guideline:

EPA 660/3-75-009: Method for acute toxicity tests with fish, macroinvertebrates and amphibians (1975)

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Named in reference as Salmo gairdneri the former name for Rainbow trout - no data given on size etc.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not applicable

Hardness:

No data given

Test temperature:

No data given

pH:

No data given

Dissolved oxygen:

No data given

Salinity:

Not aplicable

Nominal and measured concentrations:

No data given

Details on test conditions:

Test carried out according to "Committee on methods for acute toxicity tests with aquatic organisms" - Method for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians, Ecological Research Series, EPA-660/3-75-009, US EPA (1975)

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

95% confidence limit 96h LC50: 0.28-0.50 mg/L

Sublethal observations / clinical signs:

95% confidence limit 96h LC50: 0.28-0.50 mg/L (0.4mg/L)

Conclusions:

The LC50 for rainbow trout has been determined as 0.4 mg/L

Executive summary:

The study was performed according to EPA 660/3-75-009: Method for acute toxicity tests with fish,  macroinvertebrates and amphibians (1975) to assess the acute toxicity of triphenyl phosphate to Oncorhynchus mykisss under static conditions. The LC 50 after 96 hours was calculated as 0.4 mg/L.

Description of key information

Mayer et al. (1981) reports static test results with 96 h-LC50 values of 0.4 mg/L for Oncorhynchus mykiss and 0.32 - 0.56 mg/L for the marine species Cyprinodon variegatus (both nominal concentrations).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.4 mg/L

Marine water fish

Marine water fish

Effect concentration:

0.32 mg/L

Additional information

Several acute fish tests were performed according to the U.S. guideline EPA-660/3-75-009. Mayer et al. (1981) reports static test results with 96 h-LC50 values of 0.4 mg/L for Oncorhynchus mykissand 0.32 - 0.56 mg/L for the marine species Cyprinodon variegatus (both nominal concentrations). Further acute fish toxicity data were generated in studies on killifish (Oryzias latipes) and goldfish (Carassius auratus) (Sasaki et al, 1982), Oncorhynchus mykiss(Palawski et al, 1983) and Lepomis macrochirus (Huckins et al, 1991). These studies reported LC50 values that are broadly comparable with those reported in the key study by Mayer et al. (1981) presented here.