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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro DNA damage and/or repair study
Remarks:
Type of genotoxicity: DNA damage and/or repair
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: detailed description of testsystem given. Also assessed by OECD.

Data source

Reference
Reference Type:
other: Thesis
Title:
Beziehungen zwischen neurotoxischer, mutagener und kanzerogener Wirkung organischer Phosphorverbindungen - Entwicklung und Validierung eines Zellkulturverfahrens zur Voraussage neurotoxischer Effekte.
Author:
Schmuck G
Year:
1989
Bibliographic source:
Thesis, University of Würzburg, Germany

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 482 (Genetic Toxicology: DNA Damage and Repair, Unscheduled DNA Synthesis in Mammalian Cells In Vitro)
GLP compliance:
not specified
Type of assay:
DNA damage and repair assay, unscheduled DNA synthesis in mammalian cells in vitro

Test material

Constituent 1
Chemical structure
Reference substance name:
Triphenyl phosphate
EC Number:
204-112-2
EC Name:
Triphenyl phosphate
Cas Number:
115-86-6
Molecular formula:
C18H15O4P
IUPAC Name:
triphenyl phosphate

Method

Species / strain
Species / strain / cell type:
other: Syrian hamster embryonic fibroblast cells (SHE)
Metabolic activation:
without
Test concentrations with justification for top dose:
0.1 - 10x E-5 M
Details on test system and experimental conditions:
Unscheduled DNA synthesis

Results and discussion

Test results
Species / strain:
other:
Metabolic activation:
without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: Test system: Syrian hamster embryonic fibroblast cells (SHE)

Applicant's summary and conclusion

Conclusions:
Interpretation of results: negative without metabolic activation

TPP was negative in inducing unscheduled DNA synthesis.
Executive summary:

Unscheduled DNA synthesis was examined in Syrian hamster fibroblast cells at concentrations of 0.05 to 10 x 10 -5 M. After 5 hours of incubation without metabolic activation in the presence of 3H-thymidine. TPP showed no genotoxic effect.