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EC number: 204-112-2 | CAS number: 115-86-6
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Biodegradation in water and sediment: simulation tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: sewage treatment simulation testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study (also assessed by OECD)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units)
- Deviations:
- yes
- Remarks:
- for the determination of the DOC-analysis on the carbon analyzer a 0.45 µm filter was used instead of the 1.2 µm filter; The concentration was 5 mg/L test substance instead 20 mg DOC/L
- GLP compliance:
- yes
- Radiolabelling:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: synthetic mixture of activated sludge, from sewage treatment plant, river, and garden soil, adapted
- Details on inoculum:
- Activated sludge was produced in the test apparatus from a mixed inoculum obtained from a sewage treatment plant, river water and garden soil. Adaptation period 14 days at 23±2C
- Duration of test (contact time):
- 20 d
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- other: Amount of parent determined by GC or HPLC
- Details on study design:
- The test was carried out in an all glass Husmann-Apparatus with a 3 litre aeration vessel. A stock solution of TPP was prepared by emulsifying 125 mg TPP in 1 ml octanol, 0.25 ml Tween and 1000ml bidistilled water and applying ultrasound during 30 seconds; nutrient solution was prepared fresh every day. The average retention time in the aeration vessel was 3 h by continuous feeding of the solutions at a rate of 1 litre/h for 24h per day; temperature 23±2°C; pH: 7.4 - 8.2, measurement of dissolved oxygen (7.0 - 8.9 mg/l), DOC, and TPP; analysis of sludge at the end of the test for accumulated TPP. A blank test was set up using nutrient solution not containing TPP.
- % Degr.:
- 93.8
- Parameter:
- other: LC/GC
- Sampling time:
- 20 d
- Transformation products:
- not measured
- Volatile metabolites:
- not measured
- Residues:
- yes
- Details on results:
- 6.3% triphenyl phosphate measured in effluent plus sludge.
- Conclusions:
- Interpretation of results: inherently biodegradable
- Executive summary:
The study was conducted for 20 d according to OECD 303 A (Simulation Test -Aerobic Sewage Treatment: Coupled Units Test). The mean of the elimination rate was determined with 93.8 % of the initial TPP concentration. Less than 1 % of the total applied TPP was found in the sludge. Thus triphenyl phosphate is shown to be inherently biodegradable.
Reference
The mean of the elimination rate was determined with 93.8 % of the initial TPP concentration. Less than 1 % of the total applied TPP was found in the sludge.
Description of key information
Triphenyl phosphate is shown to be inherently biodegradable with a degradation rate of 93.8 % in 20 d by adapted sludge.
Key value for chemical safety assessment
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