Reaction mass of divinylbenzene and ethylstyrene

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals

Deviations:

no

Remarks:

Not specified

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of divinylbenzene and ethylstyrene

EC Number:

910-757-7

Cas Number:

N/A

Molecular formula:

Divinylbenzene: C10H10 Ethylstyrene: C10H12

IUPAC Name:

Reaction mass of divinylbenzene and ethylstyrene

Details on test material:

Source: Nippon Steel Chem.
Purity; 96.2%

Method

Target gene:

TA100- hisG46 , TA1535- hisG46, TA98- hisD3052, TA1537-hisC3076, Escherichia coli WP2uvr A- trp

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Metabolic activation system:

S9 mix: Rat liver, induced with phenobarbital and 5.6-benzoflavone

Test concentrations with justification for top dose:

-S9 mix; 0, 0.781, 1.56, 3.13, 6.25, 12.5, 25.0, 50.0 ug/plate (TA1535 (Test 1));
0, 1.56 - 50.0 ug/plate (TA1535 (Test 2));
0, 3.13 - 100 ug/plate (TA100, TA98, TA1537);
0, 6.25 - 200 ug/plate (WP2 uvrA)+S9 mix;
0, 6.25 -200ug/plate (TA100, TA1535, TA98, TA1537)

Vehicle / solvent:

No data

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

Positive controls:

not specified

Details on test system and experimental conditions:

-S9 mix; 0, 0.781, 1.56, 3.13, 6.25, 12.5, 25.0, 50.0
ug/plate (TA1535 (Test 1)); 0, 1.56 - 50.0 ug/plate (TA1535
(Test 2)); 0, 3.13 - 100 ug/plate (TA100, TA98, TA1537); 0,
6.25 - 200 ug/plate (WP2 uvrA)+S9 mix; 0, 6.25 -200ug/plate
(TA100, TA1535, TA98, TA1537)

S9: Rat liver, induced with phenobarbital and
5.6-benzoflavone.
Plate/test; 3
Number of replicates; 2

Evaluation criteria:

No data

Statistics:

No data

Results and discussion

Test results

Test results 1

Key result

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Positive controls validity:

not specified

Test results 2

Key result

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

Toxicity was observed at 50.0 ug/plate (TA100, TA1535, TA1537) and 100 ug/plate (TA98, WP2 urvA) without an S9 mix, and at 100 ug/plate (TA100, TA1535, TA98, TA1537) and 250 ug/plate (WP2 urvA) with an S9 mix.

Any other information on results incl. tables

The authors concluded that DVB was negative in this reverse mutation test, although styrene oxide which was structurally related to DVB was reported to be positive in the bacterial reverse mutation test and in the chromosomal aberration test, and negative in the micronucleus test on mice.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

DVB did not induce gene mutations in S. typhimurium or E. coli strains with and without metabolic activation

Executive summary:

The study was conducted according to the Guidelines for screening mutagenicity testing of chemicals, JAPAN. DVB did not induce gene mutations in S. typhimurium or E. coli strains with and without metabolic activation.