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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1998
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1998

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
Deviations:
no
Remarks:
Not specified
Principles of method if other than guideline:
Not applicable
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction mass of divinylbenzene and ethylstyrene
EC Number:
910-757-7
Cas Number:
N/A
Molecular formula:
Divinylbenzene: C10H10 Ethylstyrene: C10H12
IUPAC Name:
Reaction mass of divinylbenzene and ethylstyrene
Details on test material:
Source: Nippon Steel Chem.
Purity; 96.2%

Method

Target gene:
TA100- hisG46 , TA1535- hisG46, TA98- hisD3052, TA1537-hisC3076, Escherichia coli WP2uvr A- trp
Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Species / strain / cell type:
E. coli WP2 uvr A
Metabolic activation:
with and without
Metabolic activation system:
S9 mix: Rat liver, induced with phenobarbital and 5.6-benzoflavone
Test concentrations with justification for top dose:
-S9 mix; 0, 0.781, 1.56, 3.13, 6.25, 12.5, 25.0, 50.0 ug/plate (TA1535 (Test 1));
0, 1.56 - 50.0 ug/plate (TA1535 (Test 2));
0, 3.13 - 100 ug/plate (TA100, TA98, TA1537);
0, 6.25 - 200 ug/plate (WP2 uvrA)+S9 mix;
0, 6.25 -200ug/plate (TA100, TA1535, TA98, TA1537)
Vehicle / solvent:
No data
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
Positive controls:
not specified
Details on test system and experimental conditions:
-S9 mix; 0, 0.781, 1.56, 3.13, 6.25, 12.5, 25.0, 50.0
ug/plate (TA1535 (Test 1)); 0, 1.56 - 50.0 ug/plate (TA1535
(Test 2)); 0, 3.13 - 100 ug/plate (TA100, TA98, TA1537); 0,
6.25 - 200 ug/plate (WP2 uvrA)+S9 mix; 0, 6.25 -200ug/plate
(TA100, TA1535, TA98, TA1537)

S9: Rat liver, induced with phenobarbital and
5.6-benzoflavone.
Plate/test; 3
Number of replicates; 2
Evaluation criteria:
No data
Statistics:
No data

Results and discussion

Test resultsopen allclose all
Key result
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Positive controls validity:
not specified
Key result
Species / strain:
E. coli WP2 uvr A
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
Toxicity was observed at 50.0 ug/plate (TA100, TA1535, TA1537) and 100 ug/plate (TA98, WP2 urvA) without an S9 mix, and at 100 ug/plate (TA100, TA1535, TA98, TA1537) and 250 ug/plate (WP2 urvA) with an S9 mix.

Any other information on results incl. tables

The authors concluded that DVB was negative in this reverse mutation test, although styrene oxide which was structurally related to DVB was reported to be positive in the bacterial reverse mutation test and in the chromosomal aberration test, and negative in the micronucleus test on mice.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

DVB did not induce gene mutations in S. typhimurium or E. coli strains with and without metabolic activation
Executive summary:

The study was conducted according to the Guidelines for screening mutagenicity testing of chemicals, JAPAN. DVB did not induce gene mutations in S. typhimurium or E. coli strains with and without metabolic activation.

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