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EC number: 204-464-7 | CAS number: 121-32-4
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Study well described, and meets generally the strandard guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Study done according to NTP standard protocols
- GLP compliance:
- not specified
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 3-ethoxy-4-hydroxybenzaldehyde
- EC Number:
- 204-464-7
- EC Name:
- 3-ethoxy-4-hydroxybenzaldehyde
- Cas Number:
- 121-32-4
- Molecular formula:
- C9H10O3
- IUPAC Name:
- 3-ethoxy-4-hydroxybenzaldehyde
- Details on test material:
- no data
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- B6C3F1
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- No more information on animals. No data on environmental conditions.
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- - Vehicle(s)/solvent(s) used: corn oil
- Justification for choice of solvent/vehicle: no data
- Concentration of test material in vehicle: no data - Duration of treatment / exposure:
- 72 hours
- Frequency of treatment:
- Daily during 3 days
- Post exposure period:
- 24 hours after last dosing.
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0; 47; 94; 187; 375; 750 mg/kg in corn oil
Basis:
nominal conc.
- No. of animals per sex per dose:
- 5 males per dose
- Control animals:
- yes, concurrent no treatment
- Positive control(s):
- none
Examinations
- Tissues and cell types examined:
- bone marrow
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION: Doses extend up to the maximum tolerated dose.
TREATMENT AND SAMPLING TIMES ( in addition to information in specific fields): Based on the cell cycle and maturation times of the erythrocytes, harvesting of the bone marrow usually occurs 24 hours after the last dosing; this interval is indicated in the data tables as "sample collection time."
DETAILS OF SLIDE PREPARATION: The bone marrow is flushed from the femurs and spread onto slides. The slides are air-dried, fixed, and stained with a fluorescent DNA-specific stain that easily illuminates any micronuclei that may be present.
METHOD OF ANALYSIS: no data - Evaluation criteria:
- Typically, 2000 polychromatic erythrocytes (PCEs, reticulocytes; immature erythrocytes) are scored per animal for frequency of micronucleated cells in each of 5 animals per dose group. In addition, the percentage of PCEs among the total erythrocyte population in the bone marrow is scored for each dose group as an indicator of chemical-induced toxicity. In non-treated healthy mice and rats, the %PCE in bone marrow is usually around 50-60%. If a chemical interferes with the production of erythrocytes in the bone marrow, then the %PCE in the bone marrow may decline from the typical normal level. Conversely, if erythrocyte production is stimulated by chemical exposure, then a higher percentage of immature erythrocytes may be observed.
- Statistics:
- no data
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- not specified
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
Applicant's summary and conclusion
- Conclusions:
- negative
- Executive summary:
In the NTP report (1984), male mice (5 per dose) were exposed by i.p. route to ethylvanillin at doses 0; 47; 94; 187; 375; 750 mg/kg in corn oil for 72 hours. 2000 polychromatic erythrocytes were scored per animal for frequency of micronucleated cells.
Negative result was reported.
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