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EC number: 204-464-7 | CAS number: 121-32-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Remarks:
- The test conditions are well described and are in accordance with the recognised EC and OECD test guidelines. The GLP are not mentioned.
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute toxicities of organic chemicals to fathead minnow (Pimephales promelas)
- Author:
- Brooke LT , Call DJ, Geiger DL and Northcott CE
- Year:
- 1 984
- Bibliographic source:
- Center for lake Superior Environmental Studies 1984, vol I: 327-328
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 3-ethoxy-4-hydroxybenzaldehyde
- EC Number:
- 204-464-7
- EC Name:
- 3-ethoxy-4-hydroxybenzaldehyde
- Cas Number:
- 121-32-4
- Molecular formula:
- C9H10O3
- IUPAC Name:
- 3-ethoxy-4-hydroxybenzaldehyde
- Details on test material:
- from
Aldrich Chemicals Co
Purity : 97 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Toxicants were proportionally diluted with lake superior water from stock solution before delivery to fish exposure chamber.
Test chemicals were prepared for proportional dilution by the diluted stock method. i e : premixed aqueous solution of desired concentration.
Stock solution: 1000 mg/L
- Controls: blank
Test organisms
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Pimephales promelas (Fathead minnow)
- Source: From US EPA Environmental Research Laboratory- Duluth
- Age at study initiation: 28 days
- Weight at study initiation: Mean: 0.034 g; SD: 0.0131 g
- Length at study initiation: Mean: 14.3 mm; SD: 2.245 mm
- Method of breeding: Adults were held at 25°C in flowing water with a 16-h light-controlled photoperiod and fed frozen adult brine shrimp. They were provided with asbestos pipe (cut in half longitudinally) as spawning substrate. Eggs were spawed and fertilized on the undersides of these substrates. The embryos were tended by the male parent until eye-up at which time the substrates with embryos attached were removed to another 25°C bath where hatching occured.
- Feeding during test: no
ACCLIMATION
- Acclimation period: no
QUARANTINE: no data
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- behaviour also reported during the test
Test conditions
- Hardness:
- 52.2 mg/L CaCO3 (SD: 0.65) measured by the compleximetric or EDTA method
- Test temperature:
- 24.1 °C (SD: 0.83) measured daily using partial immersion of a mercury thermometer
- pH:
- 7.69 (SD: 0.28) measured with a meter calibrated shortly prior each test
- Dissolved oxygen:
- 6.5 mg/L (SD: 0.41) measured with an oxygen sensitive electrode calibrated weekly using the azide modification of the winkley method
- Salinity:
- no data (fresh water)
- Nominal and measured concentrations:
- nominal concentration : 0 / 12.5 / 25 / 50 / 100 / 200 /
measured concentrations : see table 1 in "any other information" - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 3.4 l glass open tank
- Aeration: no
- Type of flow-through: cycling proportional diluters
- Renewal rate of test solution: no data
- No. of organisms per vessel: 22 to 26 fish per tank
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 0
WATER PARAMETERS:
- Source: Lake Superior water
- Total organic carbon:
- Metals: Al (1-26 µg/L); Cd (<0.1 µg/L); Cr (2-20 µg/L); Co (<0.5 µg/L); Cu (0.3-3.2 µg/L); Fe (2-83 µg/L); Pb (7-20 µg/L); Mn (0.2-11.5 µg/L); Ni (<0.5 µg/L); Zn (1-2.7 µg/L)
- Intervals of water quality measurement: at least once for each test.
TEST MEDIUM PARAMETERS:
- Test temperature: 24.1 °C
- dissolved oxygen: 6.5 mg/l
- hardness (CaCO3): 52.5 mg/L
- pH: 7.69
OTHER TEST CONDITIONS: no data
photoperiod : no data
EFFECT PARAMETERS MEASURED: mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 0, 12.5, 25, 50, 100 and 200 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 87.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 81.4 - 94.3
- Details on results:
- Affected fish swan at surface. Respiration and gill movements were limited. Fish were also hypoactive and lost equilibrium prior to death.
- Results with reference substance (positive control):
- not relevant
- Reported statistics and error estimates:
- no data
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table 2: Mortalities observed in each exposure chamber
Hour
control
A
B
C
D
E
Initial n° of fish in each exposure chamber
26
25
25
25
23
22
26
24
25
26
25
25
1.75
1
0
0
1
1
0
1
0
0
1
3
2
3
1
0
0
1
1
0
1
0
0
1
3
4
6
1
0
0
1
1
0
1
0
0
1
4
6
12
1
0
0
1
1
0
2
0
0
1
21
20
24
1
0
0
1
2
0
3
0
0
1
22
22
29.5
1
0
0
1
2
0
3
0
0
1
22
22
48
1
0
0
1
2
0
3
0
3
1
24
24
53.5
1
0
0
1
2
0
3
0
3
1
24
24
72
1
0
0
1
2
0
3
0
3
1
25
25
77.2
1
0
0
1
2
0
3
0
3
1
25
25
96
1
0
0
1
2
1
3
0
3
1
25
25
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- With an EC50-96h = 87.6 mg/L for Pimephales promelas, ethylvanillin is harmful for fish.
The validity criteria were not examined by the authors but 3 of the 4 validity criteria detailled in 203 OECD guideline were fullfilled:
- mortality in the controls did not exceed 10% at the end of the test
- the test was conducted under flow through conditions
- deviation of measured concentration from nominal ones was greater than 20% BUT the results were based on measured concentrations.
Concerning oxygen concentration, it is described that "test mean concentration were maintained between 60 and 100 % of water saturation except in 12 tests" it is not clear if it is the case for ethylvanillin test. - Executive summary:
In a 96-h acute toxicity study (Brooke, 1984), Fathead Minnows (Pimephales promelas) were exposed to ethyl vanillin at nominal concentrations of 0 (control); 12.5; 25; 50; 100 & 200 mg./L under flow through conditions.
The 96-h LC50 was 87.6 mg a.i./L. 95% C.I.: 81.4 to 94.3 mg./L (based on measured concentrations)
According to directive 67/548/CEE, Ethylvanillin is considered as harmful for Pimephales promelas in the conditions tested.
Although GLP were not mentionned and validity criteria not detailled, this toxicity study satisfies the guideline requirement and is therefore considered as reliable with restrictions.
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