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EC number: 204-464-7
CAS number: 121-32-4
Measurement of Vanillin concentrations
showed that removal of Vanillin from the solution occured between the
replacements of solutions. The rate of removal was higher in the second
and third week of the test than in the first week. The most probable
mechanism of removal is microbial degradation. Adapted bacteria may have
developed in the solution and were transfered with the parent animals at
replacement. TheBOD5/COD ratio (0.72) confirms that
Vanillin is readily biodegradable. The contribution of accumulation of
Vanillin in Daphnia is supposed to be of minor importance for the
removal between replacement.
Vanillin has been tested on the
reproduction of Daphnia magna for 21 days according to the OECD202 and
the GLP (NIVA, 1996). Test solutions were renewed 3 times per week.
Vanillin concentration of 100 mg/L resulted in a lethal effect starting
at day 3. No lethality was observed at 56 mg/L and lower concentrations
during 21 days exposure. Sublethal effects of Vanillin included delayed
occurence of the first brood, aborted broods and reduced number of live
offspring. The EC50 for effect of reproduction (21 days) was estimated
at 24 mg/L, LOEC was 18 mg/L and NOECat 10mg/L (all nominal
concentartions). Since exposure concentrations were fluctuating, weighed
mean concentrations were calculated and led to measured EC50 for effect
on reproduction was 16 mg/L, NOEC at 5.9 mg/L and LOEC at 10mg/L.
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