3-ethoxy-4-hydroxybenzaldehyde

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Although GLP were not mentioned, and validity criteria as well as details on results were not available, the study was performed according the OECD test guideline and was generated by the Japanese Competent Authorities.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

not specified

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

mixture of sewage, soil and natural water

Details on inoculum:

Preparation of activated sludge: A mix of old and fresh activated sludge is performed every three months. Fresh activated sludge is a mixture of ten samples from different cities and industrial sewage plants, soils and rivers in Japan. This mixture was then cultured at pH=7+/-1 under aeration
Concentration: 30 mg/L
The study was performed according the OECD test guideline.

Duration of test (contact time):

14 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

other: BOD

Details on study design:

TEST CONDITIONS according the OECD test guideline.

TEST SYSTEM
- Volume of test solution: 300 mL
- Number of culture flasks/concentration: 3
- magnetic stirrer
- Test temperature: 25°C +/- 1°C
- Culture medium: according to Japanese Industrial Standards JIS K 0102-1986-21 adjusted to pH = 7
No other data

SAMPLING according the OECD test guideline.

CONTROL AND BLANK SYSTEM
- Inoculum blank: 1 test vessel with 300mL of basal culture medium (sludge)
- water + test substance at 100 mg/L
- reference substance: sludge + aniline at 100 mg/L

Reference substance:

aniline

Parameter:

other: BOD

Value:

88

Sampling time:

14 d

Key result

Parameter:

% degradation (TOC removal)

Value:

97

Sampling time:

14 d

Key result

Parameter:

% degradation (test mat. analysis)

Value:

100

Sampling time:

14 d

Remarks on result:

other: by HPLC

Results with reference substance:

With aniline, percentages of biodegradation calculated by BOD value were beyond 40% and 60% after 7 and 14 days respectively.

- Calculation of percentage of biodegradation by BOD = [ (BOD for test substance - BOD in blank) / ThOD ] * 100 - Calculation of percentage of biodegradation by TOC = [ ( DOC_w - DOC_s ) / DOC_w ] * 100 with : DOC_w : Residual amount of TOC in (water + test substance) flask DOC_s : Residual amount of TOC in (sludge + tests substance) flask - Calculation of percentage of biodegrataion by analysis of the test substance (HPLC measurments) = [ (S_w - S_s) / Sw ] * 100 with : S_w : residual amount of tests substance in (sludge + tests substanc) flask S_s : residual amount of test substance in (water + test substance ) flask

Validity criteria fulfilled:

not specified

Remarks:

The report does not indicate any deviation from the validity criteria of the OECD 301C test.

Interpretation of results:

readily biodegradable

Conclusions:

According to 301C OECD guideline, ethyl vanilline is readily biodegradble

Executive summary:

During 14 days, the biodegradation of ethyl vanillin at an initial concentration of 100 mg/L was studied according to OECD guideline 301C.

Inoculum used in the test was an activated sludge at a concentration of 30 mg/L obtained by mixing 10 different samples from different cities and industrial sewage plants, soils and rivers in Japan.

The percentage of biodegradation were estimated by following 3 parameters: the Biological Oxygen Demand (BOD), the Total Organic Carbon (TOC) and the test substance analysis (estimated by HPLC).

The corresponding percentages of biodegradation obtained after 14 days were 88 %, 98 % and 100% respectively.

Under the test conditions Ethyl vanillin is readily biodegradable.

Tthis study was performed according to OECD test guideline and was generated by the Japanese Competent Authorities. The report does not indicate any deviation from the validity criteria of the OECD 301C test.This study is therefore considered as reliable with acceptable restrictions.

Description of key information

Ethylvanillin is readily biodegradable in a MITI test (OECD 301C)

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater

Additional information

Only one experimental study was available and was selected as key study and its summary is the following:

During 14 days, the biodegrataion of ethylvanillin at an initial concentration of 100 mg/L was studied according to OECD guideline 301C.

Inoculum used in the test was an activated sludge at a concentration of 30 mg/L obtained by mixing 10 different samples from different cities and industrial sewage plants, soils and rivers in Japan.

The percentage of biodegradation were estimated by following 3 parameters: the Biological Oxygen Demand (BOD),the Total Organic Carbon (TOC) and the test substance analysis (estimated by HPLC).

The corresponding percentages of biodegradation obtained after 14 days were 88%, 98% and 100% respectively.

Under the test conditions Ethylvanillin is readily biodegradable.

Although GLP were not mentionned, this study was performed according to OECD test guideline and was generated by the Japanese Competent Authorities. The report does not indicate any deviation from the validity criteria of the OECD 301C test. This study is therefore considered as reliable with acceptable restrictions.

 

The calculation model Biowin v4.10 (EPA), showed that Ethylvanillin is considered as readily biodegradable. It was selected as supporting study.