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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Although GLP were not mentionned, and validity criteria aa well as details on results were not available, the study was performed according the OECD test guideline and was generated by the Japanese Competent Authorities.
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
not specified
GLP compliance:
not specified
Oxygen conditions:
aerobic
Inoculum or test system:
mixture of sewage, soil and natural water
Details on inoculum:
Preparation of activated sludge: A mix of old and fresh activated sludge is performed every three months. Fresh activated sludge is a mixture of ten samples from different cities and industrial sewage plants, soils and rivers in Japan. This mixture was then cultured at pH=7+/-1 under aeration
Concentration: 30 mg/L
No other information on the inoculum available.
Duration of test (contact time):
14 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
other: BOD
Details on study design:
TEST CONDITIONS: no data
TEST SYSTEM
- Volume of test solution: 300 mL
- Number of culture flasks/concentration: 3
- magnetic stirrer
- Test temperature: 25°C +/- 1°C
- Culture medium: according to Japanese Industrial Standards JIS K 0102-1986-21 adjusted to pH = 7
No other data

SAMPLING: No data

CONTROL AND BLANK SYSTEM
- Inoculum blank: 1 test vessel with 300mL of basal culture medium (sludge)
- water + test substance at 100 mg/L
- reference substance: sludge + aniline at 100 mg/L


STATISTICAL METHODS: no data
Reference substance:
aniline
Parameter:
other: BOD
Value:
88
Sampling time:
14 d
Parameter:
% degradation (TOC removal)
Value:
97
Sampling time:
14 d
Parameter:
% degradation (test mat. analysis)
Value:
100
Sampling time:
14 d
Remarks on result:
other: by HPLC
Results with reference substance:
With aniline, percentages of biodegradation calculated by BOD value were beyond 40% and 60% after 7 and 14 days respectively.

- Calculation of percentage of biodegradation by BOD = [ (BOD for test substance - BOD in blank) / ThOD ] * 100 - Calculation of percentage of biodegradation by TOC = [ ( DOCw - DOCs ) / DOCw ] * 100 with : DOCw : Residual amount of TOC in (water + test substance) flask DOCs : Residual amount of TOC in (sludge + tests substance) flask - Calculation of percentage of biodegrataion by analysis of the test substance (HPLC measurments) = [ (Sw - Ss) / Sw ] * 100 with : Sw : residual amount of tests substance in (sludge + tests substanc) flask Ss : residual amount of test substance in (water + test substance ) flask

Validity criteria fulfilled:
not specified
Interpretation of results:
readily biodegradable
Conclusions:
According to 301C OECD guideline, ethyl vanilline is readily biodegradble
Executive summary:

During 14 days, the biodegradation of ethyl vanillin at an initial concentration of 100 mg/L was studied according to OECD guideline 301C.

Inoculum used in the test was an activated sludge at a concentration of 30 mg/L obtained by mixing 10 different samples from different cities and industrial sewage plants, soils and rivers in Japan.

The percentage of biodegradation were estimated by following 3 parameters: the Biological Oxygen Demand (BOD), the Total Organic Carbon (TOC) and the test substance analysis (estimated by HPLC).

The corresponding percentages of biodegradation obtained after 14 days were 88 %, 98 % and 100% respectively.

Under the test conditions Ethyl vanillin is readily biodegradable.

Although GLP were not mentionned, and validity criteria as well as details on results were not available, this study was performed according to OECD test guideline and was generated by the Japanese Competent Authorities. This study is therefore considered as reliable with acceptable restrictions.

Description of key information

Ethylvanillin is readily biodegradable in a MITI test (OECD 301C)

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Only one experimental study was available and was selected as key study and its summary is the following:

During 14 days, the biodegrataion of ethyl vanillin at an initial concentration of 100 mg/L was studied according to OECD guideline 301C.

Inoculum used in the test was an activated sludge at a concentration of 30 mg/L obtained by mixing 10 different samples from different cities and industrial sewage plants, soils and rivers in Japan.

The percentage of biodegradation were estimated by following 3 parameters: the Biological Oxygen Demand (BOD),the Total Organic Carbon (TOC) and the test substance analysis (estimated by HPLC).

The corresponding percentages of biodegradation obtained after 14 days were 88 %, 98 % and 100% respectively.

Under the test conditions Ethyl vanillin is readily biodegradable.

Although GLP were not mentionned, and validity criteria as weel as details on results were not available, this study was performed according to OECD test guideline and was generated by the Japanese Competent Authorities. This study is therefore considered as reliable with acceptable restrictions.

The calculation model Biowin v4.10 (EPA), showed that Ethylvanillin is considered as readily biodegradable. It was selected as supporting study.