3-ethoxy-4-hydroxybenzaldehyde

Specific investigations: other studies

Administrative data

Endpoint:

cytotoxicity

Type of information:

experimental study

Adequacy of study:

weight of evidence

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Inhibitory effects of flavourings on mutagenesis induced by chemicals in bacteria.

Author:

Ohta T, Watanabe M, Watanabe K, Shirasu Y and Kada T

Year:

1986

Bibliographic source:

Food and Chemical Toxicology, 24(1), 51-54

Materials and methods

Test guideline

Qualifier:

no guideline followed

Deviations:

not applicable

Principles of method if other than guideline:

Two bacterial strains were used: E. Coli (uvrA, trpE) and salmonella thyphimurium TA98 (uvrB, hisD).
In brief, bacterial cultures were washed twice and treated with one of the mutagens in phosphate buffer for 15-60 min, after which the cells were washed again to remove the mutagen and then assayed for numbers of mutants and viable cells. Mutagen-treated cells were spread onto semi-enriched
minimal (SEM) agar plates containing a flavouring at an appropriate concentration or onto control plates containing no flavouring.
For E. Coli, the SEM agar medium consisted of Vogel-Bonner E medium supplemented with 0.4% glucose, 1.5% agar and 5% (v/v) liquid nutrient broth, while the supplement for S. Thyphimurium were 0.4% glucose, 1.5% agar and 50 µM biotin.
After appropriate dilution of the bacterial suspension, viable cells were determined on the SEM plates. Plates were incubated at 37°C for 3 days, and
then colonies of trp+ or his+ revertants and viable cells were counted.

GLP compliance:

not specified

Type of method:

in vitro

Test material

Test material information

Constituent 1

Reference substance name:

3-ethoxy-4-hydroxybenzaldehyde

EC Number:

204-464-7

EC Name:

3-ethoxy-4-hydroxybenzaldehyde

Cas Number:

121-32-4

Molecular formula:

C9H10O3

IUPAC Name:

3-ethoxy-4-hydroxybenzaldehyde

Details on test material:

no data

Results and discussion

Details on results:

Ethylvanillin showed a clear antimutagenic effect on 4-NQO-induced mutagenesis in E. Coli.
A pronounced decrease in the number of trp+ revertant colonies was observed on the plates containing ethylvanillin (50-150 µg/ml).
There was no effect with S. thyphimurium TA98.

Applicant's summary and conclusion

Conclusions:

antimutagenic effects of ethyl vanillin on 4-NQO-induced mutagenesis in E. Coli.