8-Chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid

Toxicity to soil microorganisms

Administrative data

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)

Version / remarks:

2000

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 217 (Soil Microorganisms: Carbon Transformation Test)

Version / remarks:

2000

Deviations:

no

GLP compliance:

yes

Test material

Test material information

1

Reference substance name:

8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid

Cas Number:

353258-35-2

Molecular formula:

C9H4ClF3N2O2

IUPAC Name:

8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid

Specific details on test material used for the study:

Test substance: IN-QEK31-011
Batch No.: SG0312574
Purity: 98.2%

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

yes

Remarks:

Quartz sand

Test organisms

Test organisms (inoculum):

soil

Study design

Total exposure duration:

28 d

Test conditions

Test temperature:

20 - 22°C

Moisture:

44 - 50% of water holding capacity

Organic carbon content (% dry weight):

1.22

Nominal and measured concentrations:

15.67 mg and 156.7 mg test item/kg soil dry weight.

Reference substance (positive control):

yes

Remarks:

Sodium chloride (tested at least once a year)

Results and discussion

Effect concentrations

Key result

Duration:

28 d

Basis for effect:

other: The test item has no effect on nitrogen transformation or respiration at concentrations up to and including 156.7 mg test item/kg soil dry weight

Results with reference substance (positive control):

In the most recent study, the reference item sodium chloride has long term influence on soil microflora and confirms the sensitivity of the test system.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test item has no effect on nitrogen transformation or respiration at concentrations up to and including 156.7 mg test item/kg soil dry weight

Executive summary:

A laboratory soil microflora study was conducted in a loamy sand soil to determine the effects of the test item on nitrogen transformation and soil respiration. This study was conducted according to OECD-Guideline for the Testing of Chemicals, Soil Microorganisms: Nitrogen Transformation Test, Guideline 216 and OECD-Guideline for the Testing of Chemicals, Soil Microorganisms: Carbon Transformation Test, Guideline 217, (21 January 2000).

The test item was directly applied to the soil (mixed with quartz sand) at nominal test concentrations of 15.67 mg and 156.7 mg test item/kg soil dry weight. The control consisted of soil mixed with quartz sand.

Deviations in nitrate levels and nitrate formation rates in soil treated with up to and including 156.7 mg test item/kg soil dry weight were low at day 44 and all rates were <25% indicating no effect occurred in nitrogen transformation. Deviations of short-term respiration in soil treated with up to and including 156.7 mg test item/kg soil dry weight were low at day 28 and all rates were < 25% indicating no effect occurred in carbon transformation.

The test item has no effect on nitrogen transformation or respiration at concentrations up to and including 156.7 mg test item/kg soil dry weight (deviations between treatments and controls for both nitrogen and carbon transformation tests were in the range of 25% deviation as specified by the OECD guidelines at day 44 and 28, respectively). The results indicate that there is a large margin of safety for the test item in microbial processes in soil.