Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report date:
2013

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 106 (Adsorption - Desorption Using a Batch Equilibrium Method)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: U.S. EPA Fate, Transport, and Transformation Guidelines. OPPTS 835.1230 Adsorption/Desorption (October 2008)
Deviations:
no
GLP compliance:
yes
Type of method:
batch equilibrium method
Media:
soil

Test material

1
Chemical structure
Reference substance name:
8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid
Cas Number:
353258-35-2
Molecular formula:
C9H4ClF3N2O2
IUPAC Name:
8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid
Specific details on test material used for the study:
Test substance: 14C-IN-QEK31
Lot #: 1572460
Radiochemical purity: 97.8%
Specific activity: 69.12 μCi/mg
Radiolabelling:
yes

Study design

Test temperature:
20 ± 2°C

Batch equilibrium or other method

Analytical monitoring:
yes
Matrix propertiesopen allclose all
Matrix no.:
#1
Matrix type:
sandy loam
% Clay:
11
% Silt:
30
% Sand:
59
% Org. carbon:
1.3
pH:
7.7
CEC:
8.8 meq/100 g soil d.w.
Bulk density (g/cm³):
1.07
Matrix no.:
#2
Matrix type:
silty clay loam
% Clay:
35
% Silt:
52
% Sand:
13
% Org. carbon:
2
pH:
6.2
CEC:
17.5 meq/100 g soil d.w.
Bulk density (g/cm³):
1.03
Matrix no.:
#3
Matrix type:
clay
% Clay:
49
% Silt:
37
% Sand:
14
% Org. carbon:
2
pH:
7.7
CEC:
16.3 meq/100 g soil d.w.
Bulk density (g/cm³):
1.03
Matrix no.:
#4
Matrix type:
sandy loam
% Clay:
11
% Silt:
36
% Sand:
53
% Org. carbon:
0.56
pH:
6.5
CEC:
14.8 meq/100 g soil d.w.
Bulk density (g/cm³):
1.07
Matrix no.:
#5
Matrix type:
loamy sand
% Clay:
7
% Silt:
15
% Sand:
78
% Org. carbon:
1.7
pH:
5.6
CEC:
8.5 meq/100 g soil d.w.
Bulk density (g/cm³):
1.18
Matrix no.:
#6
Matrix type:
sandy loam
% Clay:
7
% Silt:
30
% Sand:
63
% Org. carbon:
2.6
pH:
5.9
CEC:
8.1 meq/100 g soil d.w.
Bulk density (g/cm³):
1.04
Duration of adsorption equilibration
Duration:
24 h
Temp.:
20 °C
Remarks:
Nominal application rates: 0.01, 0.05, 0.1, 0.5, 1 μg a.i./mL for all the six soils
Duration of desorption equilibrationopen allclose all
Sample no.:
#1
Duration:
24 h
Temp.:
20 °C
Remarks:
Amount of Test Material Present in the Adsorbed State (µg): 4.34 & 4.47 for replicate 1 & 2 respectively
Sample no.:
#2
Duration:
24 h
Temp.:
20 °C
Remarks:
Amount of Test Material Present in the Adsorbed State (µg): 12.17 & 12.10 for replicate 1 & 2 respectively
Sample no.:
#3
Duration:
24 h
Temp.:
20 °C
Remarks:
Amount of Test Material Present in the Adsorbed State (µg): 7.71 & 7.61 for replicate 1 & 2 respectively
Sample no.:
#4
Duration:
24 h
Temp.:
20 °C
Remarks:
Amount of Test Material Present in the Adsorbed State (µg): 4.42 & 4.44 for replicate 1 & 2 respectively
Sample no.:
#5
Duration:
24 h
Temp.:
20 °C
Remarks:
Amount of Test Material Present in the Adsorbed State (µg): 8.15 & 8.28 for replicate 1 & 2 respectively
Sample no.:
#6
Duration:
24 h
Temp.:
20 °C
Remarks:
Amount of Test Material Present in the Adsorbed State (µg): 7.95 & 7.99 for replicate 1 & 2 respectively

Results and discussion

Adsorption coefficientopen allclose all
Sample No.:
#1
Type:
Kd
Value:
0.75 other: mL/g
Temp.:
20 °C
Matrix:
Nambsheim soil
Sample No.:
#2
Type:
Kd
Value:
4.16 other: mL/g
Temp.:
20 °C
Matrix:
Tama soil
Sample No.:
#3
Type:
Kd
Value:
1.61 other: mL/g
Temp.:
20 °C
Matrix:
Lleida soil
Sample No.:
#4
Type:
Kd
Value:
0.81 other: mL/g
Temp.:
20 °C
Matrix:
Porterville soil
Sample No.:
#5
Type:
Kd
Value:
1.74 other: mL/g
Temp.:
20 °C
Matrix:
Speyer soil
Sample No.:
#6
Type:
Kd
Value:
1.65 other: mL/g
Temp.:
20 °C
Matrix:
Sassafras soil
Sample No.:
#1
Type:
other: Kom
Value:
34 other: mL/g
Temp.:
20 °C
Matrix:
Nambsheim soil
Sample No.:
#2
Type:
other: Kom
Value:
122 other: mL/g
Temp.:
20 °C
Matrix:
Tama soil
Sample No.:
#3
Type:
other: Kom
Value:
46 other: mL/g
Temp.:
20 °C
Matrix:
Lleida soil
Sample No.:
#4
Type:
other: Kom
Value:
82.9 other: mL/g
Temp.:
20 °C
Matrix:
Porterville soil
Sample No.:
#5
Type:
other: Kom
Value:
57.9 other: mL/g
Temp.:
20 °C
Matrix:
Speyer soil
Sample No.:
#6
Type:
other: Kom
Value:
54.8 other: mL/g
Temp.:
20 °C
Matrix:
Sassafras soil
Sample No.:
#1
Type:
Koc
Value:
57.5 other: mL/g
Temp.:
20 °C
Matrix:
Nambsheim soil
Sample No.:
#2
Type:
Koc
Value:
208 other: mL/g
Temp.:
20 °C
Matrix:
Tama soil
Sample No.:
#3
Type:
Koc
Value:
80.6 other: mL/g
Temp.:
20 °C
Matrix:
Lleida soil
Sample No.:
#4
Type:
Koc
Value:
144 other: mL/g
Temp.:
20 °C
Matrix:
Porterville soil
Sample No.:
#5
Type:
Koc
Value:
102 other: mL/g
Temp.:
20 °C
Matrix:
Speyer soil
Sample No.:
#6
Type:
Koc
Value:
96.8 other: mL/g
Temp.:
20 °C
Matrix:
Sassafras soil
Partition coefficientsopen allclose all
Sample No.:
#1
Type:
other: Kd
Matrix:
Nambsheim soil
Remarks on result:
other: 0.76 & 1 mL/g for replicate 1 and 2 respectively
Sample No.:
#2
Type:
other: Kd
Matrix:
Tama soil
Remarks on result:
other: 3.66 & 4.29 mL/g for replicate 1 and 2 respectively
Sample No.:
#3
Type:
other: Kd
Matrix:
Lleida soil
Remarks on result:
other: 1.61 & 2.04 mL/g for replicate 1 and 2 respectively
Sample No.:
#4
Type:
other: Kd
Matrix:
Porterville soil
Remarks on result:
other: 0.78 & 0.97 mL/g for replicate 1 and 2 respectively
Sample No.:
#5
Type:
other: Kd
Matrix:
Speyer soil
Remarks on result:
other: 1.65 & 2.03 mL/g for replicate 1 and 2 respectively
Sample No.:
#6
Type:
other: Kd
Matrix:
Sassafras soil
Remarks on result:
other: 1.57 & 2 mL/g for replicate 1 and 2 respectively
Sample No.:
#1
Type:
other: Kdom
Matrix:
Nambsheim soil
Remarks on result:
other: 34.6 & 45.2 mL/g for replicate 1 and 2 respectively
Sample No.:
#2
Type:
other: Kdom
Matrix:
Tama soil
Remarks on result:
other: 108 & 126 mL/g for replicate 1 and 2 respectively
Sample No.:
#3
Type:
other: Kdom
Matrix:
Lleida soil
Remarks on result:
other: 45.9 & 58.3 mL/g for replicate 1 and 2 respectively
Sample No.:
#4
Type:
other: Kdom
Matrix:
Porterville soil
Remarks on result:
other: 80.5 & 99.9 mL/g for replicate 1 and 2 respectively
Sample No.:
#5
Type:
other: Kdom
Matrix:
Speyer soil
Remarks on result:
other: 55 & 67.7 mL/g for replicate 1 and 2 respectively
Sample No.:
#6
Type:
other: Kdom
Matrix:
Sassafras soil
Remarks on result:
other: 35.7 & 45.5 mL/g for replicate 1 and 2 respectively
Sample No.:
#1
Type:
other: Kdoc
Matrix:
Nambsheim soil
Remarks on result:
other: 58.5 & 76.5 mL/g for replicate 1 and 2 respectively
Sample No.:
#2
Type:
other: Kdoc
Matrix:
Tama soil
Remarks on result:
other: 183 & 215 mL/g for replicate 1 and 2 respectively
Sample No.:
#3
Type:
other: Kdoc
Matrix:
Lleida soil
Remarks on result:
other: 80.3 & 102 mL/g for replicate 1 and 2 respectively
Sample No.:
#4
Type:
other: Kdoc
Matrix:
Porterville soil
Remarks on result:
other: 140 & 173 mL/g for replicate 1 and 2 respectively
Sample No.:
#5
Type:
other: Kdoc
Matrix:
Speyer soil
Remarks on result:
other: 97.1 & 120 mL/g for replicate 1 and 2 respectively
Sample No.:
#6
Type:
other: Kdoc
Matrix:
Sassafras soil
Remarks on result:
other: 60.4 & 77 mL/g for replicate 1 and 2 respectively

Results: Batch equilibrium or other method

Adsorption and desorption constants:
The Kd values were 0.75, 4.16, 1.61, 0.81, 1.74, and 1.65 mL/g for the Nambsheim, Tama, Lleida, Porterville, Speyer and Sassafras soils, respectively.
The Kom values were 34.0, 122, 46.0, 82.9, 57.9, and 54.8 mL/g for the Nambsheim, Tama, Lleida, Porterville, Speyer and Sassafras soils, respectively.
The Koc values were 57.5, 208, 80.6, 144, 102, and 96.8 mL/g for the Nambsheim, Tama, Lleida, Porterville, Speyer and Sassafras soils, respectively.

The mean desorption Kd, values ranged from 0.76 mL/g in the Nambsheim soil to 4.29 mL/g in the Tama soil. The mean desorption Kd,om values ranged from 34.6 mL/g in the Nambsheim soil to 126 mL/g in the Tama soil. The mean desorption Kd,oc values ranged from 58.5 mL/g in the Nambsheim soil to 215 mL/g in the Tama soil.
Mass balance (%) at end of desorption phaseopen allclose all
Sample no.:
#1
Duration:
24 h
% Desorption:
99.7
Sample no.:
#2
Duration:
24 h
% Desorption:
98.5
Sample no.:
#3
Duration:
24 h
% Desorption:
96.7
Sample no.:
#4
Duration:
24 h
% Desorption:
99.8
Sample no.:
#5
Duration:
24 h
% Desorption:
96.5
Sample no.:
#6
Duration:
24 h
% Desorption:
100.9

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The analysis of adsorption supernatants conducted for the highest test concentration for each soil demonstrated that 14C-IN-QEK31 accounted between 98.24% and 100.00% of radioactivity in all test soils indicating the stability of test item during the 24-h experimental conditions.
The average material balance was quantitative and ranged from 96.5 to 100.9% of the applied radioactivity in all soils.
Executive summary:

The adsorption and desorption properties of 14C-IN-QEK31 to six different soils were investigated in the laboratory. The calculated adsorption (Kd, Kom and Koc) and desorption coefficients (Kd1, Kd2, Kd1,om, Kd1,oc, Kd2,om and Kd2,oc) and the Freundlich adsorption isotherm parameters (KF, KFom, KFoc, 1/n and R2) are reported.


The texture of the soils included three sandy loams (Nambsheim, Porterville and Sassafras), a silty clay loam (Tama), a loamy sand (Speyer) and a clay (Lleida). The per cent organic matter (Walkley-Black method) of the soils ranged from 0.97 to 4.4% and the pH in aqueous 0.01 M CaCl2 ranged from 5.3 to 7.5. The study was conducted as per procedures outlined in OECD Guideline 106 (January 2000) and U. S. EPA test guidelines OPPTS 835.1230 (October 2008). The adsorption experiment was performed using the batch equilibration method on soils with five nominal test concentrations (0.01, 0.05, 0.1, 0.5 and 1 µg/mL) of the test item prepared in aqueous 0.01 M CaCl2. The highest concentration chosen was commensurate with the likely concentration in soil at the projected application rates of 1.0 kg/ha. Two desorption cycles were performed on samples treated at the highest test concentration. A 1:2 (w/v) soil-to-solution ratio was used in the preliminary and definitive experiments. The sorption coefficients, Kd, Kom and Koc were calculated for each soil at each test concentration.


The Kd values were 0.75, 4.16, 1.61, 0.81, 1.74, and 1.65 mL/g for the Nambsheim, Tama, Lleida, Porterville, Speyer and Sassafras soils, respectively.


The Kom values were 34.0, 122, 46.0, 82.9, 57.9, and 54.8 mL/g for the Nambsheim, Tama, Lleida, Porterville, Speyer and Sassafras soils, respectively.


The Koc values were 57.5, 208, 80.6, 144, 102, and 96.8 mL/g for the Nambsheim, Tama, Lleida, Porterville, Speyer and Sassafras soils, respectively.


The values for the Freundlich adsorption isotherm parameters, KF, KFom, KFoc, 1/n and R2 were derived from the linear form of the Freundlich equation for all test soils. A plot of Kd and Koc against soil pH showed no correlation of pH with adsorption parameters. The value of the Freundlich adsorption isotherm parameter, KF, was 0.53, 2.68, 1.16, 0.55, 1.31 and 1.27 for the Nambsheim, Tama, Lleida, Porterville, Speyer and Sassafras soils, respectively. The organic matter and organic carbon normalized Freundlich adsorption isotherm coefficients KFom and KFoc, respectively, were calculated for all the soils. The KFom values were 24.1, 78.8, 33.1, 56.7, 43.7 and 28.9 while the KFoc values were 40.8, 134, 58.0, 98.2, 77.1 and 48.8 for Nambsheim, Tama, Lleida, Porterville, Speyer and Sassafras soils, respectively. The values for the Freundlich constant, 1/n, were 0.8630, 0.8693, 0.8831, 0.8550, 0.8992 and 0.9070 for Nambsheim, Tama, Lleida, Porterville, Speyer and Sassafras soils, respectively. The correlation coefficient (R2) for the analyses were 0.9996, 0.9997, 0.9998, 0.9995, 0.9991 and 0.9996 for Nambsheim, Tama, Lleida, Porterville, Speyer and Sassafras soils, respectively, for the adsorption phase, indicating the Freundlich equation adequately predicts the adsorption of the test item over the concentration range studied.


The desorption coefficients, Kd1, Kd2, Kd1,om, Kd1,oc, Kd2,om and Kd2,oc were calculated for each soil at the highest test concentration. The mean desorption Kd, values ranged from 0.76 mL/g in the Nambsheim soil to 4.29 mL/g in the Tama soil. The mean desorption Kd,om values ranged from 34.6 mL/g in the Nambsheim soil to 126 mL/g in the Tama soil. The mean desorption Kd,oc values ranged from 58.5 mL/g in the Nambsheim soil to 215 mL/g in the Tama soil.


The per cent 14C-IN-QEK31 desorbed from the soils during two desorption intervals (D1 and D2) was calculated and totaled (DT) for each soil at the highest test concentration. The total per cent desorbed (DT) values ranged from 35.2% in the Tama soil to 64.5% in the Porterville soil. In general, lower desorption values were observed in second desorption. The highest desorption was observed in Nambsheim and Porterville soils in first and second desorption, respectively. The lowest desorption was observed in Tama soil in both first and second desorption intervals.


The analysis of adsorption supernatants conducted for the highest test concentration for each soil demonstrated that 14C-IN-QEK31 accounted between 98.24% and 100.00% of radioactivity in all test soils indicating the stability of test item during the 24-h experimental conditions.


The average material balance was quantitative and ranged from 96.5 to 100.9% of the applied radioactivity in all soils.