8-Chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

1

Reference substance name:

8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid

Cas Number:

353258-35-2

Molecular formula:

C9H4ClF3N2O2

IUPAC Name:

8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid

Specific details on test material used for the study:

Test substance: IN-QEK31-011
Batch No.: SG0312574
Purity: 98.2%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Remarks:

ISO medium

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

other: ISO medium

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg/L as CaCO3

Test temperature:

20 - 22°C

pH:

6.7 - 8.0

Dissolved oxygen:

8.2 - 8.7 mg/L

Nominal and measured concentrations:

Nominal: 120, 55, 25, 11 and 5.1 mg/L
Measured: 125, 56.9, 26.2, 11.6 and 5.20 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 125 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: highest concentration tested

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 125 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: highest concentration tested

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 125 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: highest concentration tested

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

48-hour EC50 (Daphnia magna): >125 mg/L (highest concentration tested)
48-hour NOEC (Daphnia magna): ≥125 mg/L
48-hour LOEC (Daphnia magna): >125 mg/L

Executive summary:

The objective of this test was to determine the acute effects of the test item on the cladoceran Daphnia magna. Young Daphnia (< 24 hours old) were exposed in a static test to the test item for 48 hours, added to test water at a range of concentrations. Under otherwise identical test conditions, different concentrations of the test item result in different percentages of daphnids being no longer capable of swimming at the end of the test. The test method of application and the test species, Daphnia magna, are recommended by the OECD Guideline 202.

The effect of the test item on the mobility of Daphnia magna was determined in ISO medium (prepared according to OECD guideline 202). Four replicates with 5 test animals each at five nominal test concentrations of 120, 55, 25, 11 and 5.1 mg/L, corresponding to mean measured concentration of 125, 56.9, 26.2, 11.6 and 5.20 mg/L, and a test water control were incubated in a controlled room environment for 48 hours at 20 – 22 °C and the mobility of the Daphnia was determined daily.

The toxic effect of the test item to Daphnia magna was determined in a static test. The 48-hour EC50 value was determined to be higher than 125 mg/L (mean measured concentration). The 48-hour NOEC was higher than or equal to 125 mg/L (mean measured concentration) and the 48-hour LOEC was higher than 125 mg/L (mean measured concentration).