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Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014
Report date:
2014

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
Deviations:
no
Qualifier:
according to guideline
Guideline:
ISO 11268-2 (Effects of Pollutants on Earthworms. 2. Determination of Effects on Reproduction)
Deviations:
no
GLP compliance:
yes

Test material

1
Chemical structure
Reference substance name:
8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid
Cas Number:
353258-35-2
Molecular formula:
C9H4ClF3N2O2
IUPAC Name:
8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid
Specific details on test material used for the study:
Test substance: IN-QEK31-011
Batch No.: SG0312574
Purity: 98.2%

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes
Remarks:
Quartz sand

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Remarks:
10% Sphagnum-peat, 20% Kaolin clay, 69.5% fine quartz-sand and 0.5% Calcium carbonate
Total exposure duration:
56 d
Remarks:
Adults were exposed for 28 days. After 4 weeks, adults were sorted from the soil and weighed. They were not placed back into the boxes. Juveniles which had been produced in the boxes remained in the soil for another 4 weeks without further feeding.

Test conditions

Test temperature:
18.0 - 22.0°С
pH:
5.8 to 6.0 at test start and 5.8 to 5.9 at test termination
Moisture:
Initiation: 32.8% to 33.5% (equivalent to 53.7% to 54.9% of the maximum water holding capacity) Termination: 32.7% to 35.7% (equivalent to 53.6% to 58.5% of the maximum water holding capacity)
Nominal and measured concentrations:
Nominal: 6.36, 12.73, 25.46, 50.92 and 101.8 mg test item/kg dry artificial soil (corresponding to 6.25, 12.5, 25.0, 50.0 and 100 mg/kg dry artificial soil, adjusted for purity)
Reference substance (positive control):
yes
Remarks:
Carbendazim (tested once per year)

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
28 d
Dose descriptor:
LC50
Effect conc.:
> 100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: highest concentration tested
Duration:
56 d
Dose descriptor:
NOEC
Effect conc.:
50 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: overall
Duration:
56 d
Dose descriptor:
LOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: overall
Results with reference substance (positive control):
The EC50 for reproduction of the reference item in the most recent test was 1.87 mg carbendazim/kg dry artificial soil. All validation criteria were within acceptable limits indicating the validity of this test.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
28 days LC50 (Earthworm): >100 mg/kg dry artificial soil, the highest concentration tested
NOEC Earthworm (overall): 50.0 mg/kg dry soil
LOEC Earthworm (overall): 100 mg/kg dry soil
Executive summary:

The sublethal toxicity of the test item to earthworms, Eisenia fetida, was determined in a 56-day soil exposure laboratory study according to OECD 222, 2004 and ISO 11268-2, 2012. Adult earthworms were exposed to artificial soil (prepared according to OECD 222) treated with the test item to obtain the nominal concentrations of 6.36, 12.73, 25.46, 50.92 and 101.8 mg test item/kg dry artificial soil (corresponding to 6.25, 12.5, 25.0, 50.0 and 100 mg/kg dry artificial soil, adjusted for purity) and to an untreated control (quartz sand and deionized water only). Mortality and growth (body weight) of the earthworms were assessed after 28 days and the effect on reproduction (number of juveniles produced) was assessed after 56 days.


The test item had no statistically significant lethal effects or effects on growth or feeding activity of the earthworm Eisenia fetida when exposed to concentrations up to and including 100 mg/kg dry artificial soil.


Reproduction was not statistically significantly reduced up to and including 50.0 mg/kg dry artificial soil.


The overall NOEC (No-Observed-Effect Concentration) was determined to be 50.0 mg/kg dry artificial soil and the overall LOEC (Lowest-Observed-Effect Concentration) was determined to be 100 mg/kg dry artificial soil.


The LC50 after 28 days was estimated to be greater than 100 mg/kg dry artificial soil, the highest concentration tested.