8-Chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid

Toxicity to terrestrial arthropods

Administrative data

Endpoint:

toxicity to terrestrial arthropods: short-term

Remarks:

Honey bee (oral and contact)

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 213 (Honeybees, Acute Oral Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 214 (Honeybees, Acute Contact Toxicity Test)

Deviations:

no

GLP compliance:

yes

Application method:

other: Oral and Contact

Test material

Test material information

1

Reference substance name:

8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid

Cas Number:

353258-35-2

Molecular formula:

C9H4ClF3N2O2

IUPAC Name:

8-chloro-6-(trifluoromethyl)imidazo[1,2-a]pyridine-2-carboxylic acid

Specific details on test material used for the study:

Test substance: IN-QEK31-011
Batch No.: SG0312574
Purity: 98.2%

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

yes

Remarks:

50% w/v sucrose solution with 2.5% DMSO for oral toxicity and Tap water with ca. 0.5% Adhäsit® and 10% DMSO for contact toxicity

Test organisms

Test organisms (species):

Apis mellifera

Animal group:

Hymenoptera (honeybees)

Study design

Study type:

laboratory study

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

25°C

Humidity:

51 - 84%

Photoperiod and lighting:

Darkness (except during observation)

Nominal and measured concentrations:

Contact test: 100, 50, 25, 12.5 and 6.25 μg a.i./bee
Oral test: 110.0, 55.0, 27.3, 13.4 and 7.0 μg a.i./bee

Reference substance (positive control):

yes

Remarks:

Perfekthion (dimethoate a.s.)

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

LD50

Remarks:

Oral

Effect conc.:

> 110 other: μg a.i./bee

Conc. based on:

act. ingr.

Basis for effect:

mortality

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

LD50

Remarks:

Contact

Effect conc.:

> 100 other: μg a.i./bee

Conc. based on:

act. ingr.

Basis for effect:

mortality

Effect concentrations 3

Duration:

48 h

Dose descriptor:

other: NOED (Oral)

Effect conc.:

110 other: μg a.i./bee

Conc. based on:

act. ingr.

Basis for effect:

mortality

Effect concentrations 4

Duration:

48 h

Dose descriptor:

other: NOED (contact)

Effect conc.:

100 other: μg a.i./bee

Conc. based on:

act. ingr.

Basis for effect:

mortality

Results with reference substance (positive control):

The 24-hour oral and contact LD50 values for the reference item were 0.12 and 0.13 μg dimethoate/bee, respectively.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Honey bee 48 h contact LD50: >100 μg a.i./bee
Honey bee 48 h Contact NOED: 100 μg a.i./bee
Honey bee 48 h oral LD50: >110 μg a.i./bee
Honey bee 48 h Oral NOED: 110 μg a.i./bee

Executive summary:

The aim of this study was to determine the acute contact and oral toxicity of the test item to the honey bee (Apis mellifera L.) in a laboratory study. A contact test with 100, 50, 25, 12.5 and 6.25 μg a.i./bee and an oral test with 110.0, 55.0, 27.3, 13.4 and 7.0 μg a.i./bee were conducted under OECD Guidelines 213 and 214 (1998).

In the contact test dose levels of 100, 50, 25, 12.5 and 6.25 μg a.i./bee led to no mortality at test end (48 hours after application). In the water control group (tap water containing 0.5% Adhäsit) as well as in the solvent control group (tap water with 0.5% Adhäsit and 10% DMSO) 3.3% mortality occurred.

In the oral test the target dose levels of 100, 50, 25, 12.5 and 6.25 μg a.i./bee would have been achieved if each bee ingested exactly 20 mg of the treated feeding solution. Actual oral doses of 110.0, 55.0, 27.3, 13.4 and 7.0 μg a.i./bee led to mortalities of 6.7, 0.0, 3.3, 3.3 and 3.3% at test end (48 hours after application), respectively. In the water control group (50% w/v sucrose solution) 3.3% mortality was observed. No mortality occurred in the solvent control group (50% w/v sucrose solution containing 2.5% DMSO).

No test item related behavioural abnormalities occurred at any time in both toxicity tests.

The effects of the test item on the honey bee (Apis mellifera L.) were assessed in an acute contact and oral toxicity test, conducted in the laboratory. The 48 h contact LD50 – based on analytical a.i.-content – was >100 μg a.i./bee and the contact NOED was 100 μg a.i./bee. The 48 h oral LD50 – based on analytical a.i.-content – was >110 μg a.i./bee and the oral NOED was 110 μg a.i./bee.