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EC number: 202-486-1
CAS number: 96-18-4
The biodegradation in soil under aerobic conditions was studied in a
simulation test with two soil samples (silt loam and sandy loam) in a
seven day laboratory test using air dried soil samples (Anderson et al.
1991). The toxicity of the substance to soil microorganisms was first
tested in a 7-day pre-test. An infrared gas analyser was used to measure
CO2 efflux from incubated (20 °C) 50 g soil samples. Every 24 hours a
flow of moist CO2-free air was passed through the incubation jars.
Toxicity determinations were based on whether microbial respiration in
the soil had returned to control levels by the end of the 7-day
experiment. Chemical degradation of the substance was then tested with
two soils in incubation jars which were fitted with charcoal traps to
determine the amount of chemicals lost from soil to the headspace.
Chemical solution was added to the soils which were adjusted to 80 %
water holding capacity. The soils were afterwards incubated at 20 °C in
the dark. Every 24 hours the jars were flushed with a stream of cleaned
air to sample the charcoal traps and also to maintain aerobic
conditions. The soil was extracted in toto from the jars on days 0, 2,
3, 6 and 7 and the test substances was extracted with methanol to
monitor the degradation of 1,2,3-trichloropropane. The chemical was
analysed by gas chromatography. The half-life for primary degradation in
soil of 1,2,3-trichloropropane under aerobic conditions found in the two
loam samples was about 2.7 days.
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