2-phenylpropene

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study design comparable to OECD Guideline 471/472. Study in Japanese without complete English translation.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)

Deviations:

no

Principles of method if other than guideline:

Plate incorporation method; Study performed according to Guideline for Screening Mutagenicity Testing of Chemicals (Japan)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-phenylpropene

EC Number:

202-705-0

EC Name:

2-phenylpropene

Cas Number:

98-83-9

Molecular formula:

C9H10

IUPAC Name:

(prop-1-en-2-yl)benzene

Details on test material:

Purity: 99.6 %

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Metabolic activation system:

S9 mix from rat liver (induction with phenobarbital and 5,6-benzoflavone)

Test concentrations with justification for top dose:

+/-S9 mix, all strains:
0, 12.5, 25, 50, 100, 200 or 400 ug/plate

Vehicle / solvent:

DMSO

Controls

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

Positive controls:

yes

Remarks:

see below

Positive control substance:

other: see below

Details on test system and experimental conditions:

3 plates/test
2 replicates

Positive controls:

-S9 mix:
TA 100, TA 98, E. coli WP2 uvrA: 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide
TA 1535: Sodium azide
TA 1537: 9-aminoacridine

+S9 mix:
All strains: 2-aminoanthracene

Evaluation criteria:

according to OECD Guidelines No. 471 and 472

Statistics:

Yes

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

>= 200 ug/plate (+/- S9)

Vehicle controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

>= 200 ug/plate (+/- S9)

Vehicle controls validity:

valid

Positive controls validity:

valid

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
negative E. coli WP2 uvr A

The TS was not mutagenic in Salmonella typhimurium TA 100, TA 1535, TA 98, TA 1537 and Escherichia coli WP2 uvrA, with or without an exogenous metabolic activation system.

Executive summary:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100 as well as E. coli WP2 uvr A were tested in a plate incorporation assay with a study design comparable to OECD Guidelines No. 471 and 472.

The test concentrations were given as follows:

+/-S9 mix, all strains:

0, 12.5, 25, 50, 100, 200 or 400 ug/plate

Cytotoxic effects in all strains were seen at >= 200 ug/plate (+/- S9).

The TS was not mutagenic in Salmonella typhimurium TA 100, TA 1535, TA 98, TA 1537 and Escherichia coli WP2 uvrA, with or without an exogenous metabolic activation system.