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EC number: 226-378-9 | CAS number: 5384-21-4
Dose:2000 mg/ kg bodyweight
Body Weight (gram)
Body Weight Change (%)
Dose:2000 mg/kg body weight
Hour(s) - Day 0
Key: 1 = Normal
Days of Observation (0 to 14)
No mortality and morbidity
This study was performed as per OECD No.402.
Five male and five female healthy young adult rats were randomly selected and used for conducting acute dermal toxicity study. Rats free from injury and irritation of skin were selected for the study. Twenty four hours prior to dermal application of test item, approximately 10% of body surface area of each rat was clipped. A limit dose of 2000 mg/ kg body weight of test item moistened with 0.2 ml distilled water was applied by single dermal application and observed for 14 days after treatment.
On test day 0, an amount of test item moistened with 0.2 ml distilled water was applied directly on the intact skin of clipped area of rats; the surgical gauze patch was put on to the intact skin of clipped area.This gauze patch was covered with a semi-occlusive dressing.The dressing was wrapped around the abdomen and anchored with non-irritating adhesive tape.After the 24-hour application period, the dressings were removed and theskin was gently wiped with distilled water.The skin reactions were assessed.
The animals were observed daily for mortality and clinical signs, during the acclimatization period. All animals were observed for clinical signs at approximately 1, 2, 3 and 4 hours after treatment on day 0 and once daily during test days 1‑14. Mortality was recorded after application on test day 0 and twice daily during days 1-14 (at least once on the day of sacrifice). Local signs / Skin reactions were observed daily from test days 1-14 (in common with clinical signs). Body weights were recorded on day 0 (prior to application) and on day 7 and 14. All animals were necropsied and examined macroscopically.
No mortality was observed in any animal till the end of the experimental period.
No clinical signs and any skin reaction were observed throughout the experimental period in all treated animals.
The male and female animals were observed with body weight gain compared to day 0 throughout the experiment.
The external and internal gross pathological observation of all terminally sacrificed animals did not show any pathological abnormality.
Under the conditions of this; acute dermal toxicity study of test chemical in rats is as given below:
The acute dermal median lethal dose of test chemical was >2000 mg/kg body weight.Thus by considering the CLP criteria for acute toxicity rating for the chemicals, it infers that test chemical does not exhibit acute dermal toxicity i.e it is acutely non toxic to animals.
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