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Ecotoxicological information

Short-term toxicity to fish

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
data from handbook or collection of data for various chemicals
Justification for type of information:
data from handbook or collection of data
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
OECD Guideline 236 (Fish embryo acute toxicity (FET) test)
Principles of method if other than guideline:
The effect of test chemical was studied on the early life stage of zebrafish embryos.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Remarks:
study 1: DMSO
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Common name: Zebrafish
Test type:
other: Study 1: semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
Study 1: The embryos were observed for the 24 h more after total exposure duration of 96 h.
Nominal and measured concentrations:
Study 1: Nominal concentrations: 0, 0.5, 1, 2, 5, 10, 20 and 50 μM,
Details on test conditions:
Study 1:
Number of species: 20 embryos
Number of replicates 3 replicates
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.77 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 1st study
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 2nd study
Validity criteria fulfilled:
not specified
Conclusions:
The range of median lethal concentration of the test chemical was determined to be 1.77- 5.0 mg/L.
Executive summary:

Data available from various sources for test chemical has been reviewed to determine the toxicity of the test chemical on fish: the studies are as mentioned below.

The Primary study outlines the effect of test chemical on the early life stage of zebrafish. the test was conducted in accordance with OECD 236 guidelines, fish embryo toxicity test. Seven different concentrations was selected to evaluate the median lethal concentration i.e., 0.5, 1, 2, 5, 10, 20 and 50 μM. Test was conducted for the period of 120 h, in semi static conditions, and the median lethal concentration for 96 h was determined to be 1.77 mg/L (5.2µM).

The Secondary study outlines the effect of test chemical on the fish for the period of 96.0 h. the test was conducted in accordance with OECD 203 guidelines. After the exposure period the median lethal concentration was determiend to be >5 mg/L.

The toxicity range of the test chemical was determined to be 1.77 -5.0 mg/L. As per new CLP classification criteria the test chemical can be classified into aquatic chronic category 2.

Description of key information

The range of median lethal concentration of the test chemical was determined to be 1.77- 5.0  mg/L..

Key value for chemical safety assessment

LC50 for freshwater fish:
1.77 mg/L

Additional information

Data available from various sources for test chemical and read across chemical has been reviewed to determine the toxicity of the test chemical on fish: the studies are as mentioned below.

The Primary study outlines the effect of test chemical on the early life stage of zebrafish. the test was conducted in accordance with OECD 236 guidelines, fish embryo toxicity test. Seven different concentrations was selected to evaluate the median lethal concentration i.e., 0.5, 1, 2, 5, 10, 20 and 50 μM. Test was conducted for the period of 120 h, in semi static conditions, and the median lethal concentration for 96 h was determined to be 1.77 mg/L (5.2µM).

The Secondary study outlines the effect of test chemical on the fish for the period of 96.0 h. the test was conducted in accordance with OECD 203 guidelines. After the exposure period the median lethal concentration was determiend to be >5 mg/L.

The toxicity range of the test chemical was determined to be 1.77 -5.0 mg/L. As per new CLP classification criteria the test chemical can be classified into aquatic chronic category 2.