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Ecotoxicological information

Toxicity to microorganisms

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Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
Experimental data of various test chemical
Justification for type of information:
Experimental data of various test chemical from collection of data
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
other: As mentioned below
Principles of method if other than guideline:
WoE report is based on two toxicity study of microorganisms for the test chemical.
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
yes
Remarks:
Study 1: DMSO
Details on test solutions:
Study1: Stock solutions were prepared in dimethylsulfoxide (DMSO) at concentrations of 5, 10, 25, or 50 g/L.
Test organisms (species):
other: study1: Tetrahymena pyriformis study 2: Microregma sp.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
2 d
Remarks on exposure duration:
Study 2: 28 h
Test temperature:
study 1: 27±1°C
pH:
study 1: 7.35
Nominal and measured concentrations:
study 1: Stock solutions were prepared in dimethylsulfoxide (DMSO) at concentrations of 5, 10, 25, or 50 g/L.
Details on test conditions:
study 1:
Test vessel: 250-mL Erlenmeyer flasks containing 50 mL medium.
Each flask was inoculated with log-phase culture to attain a starting concentration of ~2,500 cells/ml.
Replicates: 3 or more
Reference substance (positive control):
not specified
Key result
Duration:
2 d
Dose descriptor:
other: IGC50
Effect conc.:
116.16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: 95% CI: 96-68-150-87
Duration:
28 h
Dose descriptor:
other: not specified
Effect conc.:
70 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Feeding behaviour
Remarks on result:
other: other details not known
Validity criteria fulfilled:
not specified
Conclusions:
The effect of the test chemical to aquatic micro organisms was in a range of 70- 116.16 mg/L.
Executive summary:

Data available from various sources for target chemical has been reviewed to determine the toxicity of the test chemical on microorganisms. The studies are as mentioned below:

Data from the peer reviewed journal outlines that, The microbial growth was evaluated using the tetrahymena pyriformis population growth assay. The test was conducted in the static conditions for the period of 48 hours. Based on the outcomes the median inhibitory growth concentration was determied to be 116.16 mg/L.

Another study from the secondary source, summarizes the effect of test chemical on the microregma sp. a ciliated protozoan. Based on the feeding behaviour the effective concentration was determied to be 70 mg/L.

The effect of the test chemical to aquatic micro organisms was in a range of 70- 116.16 mg/L.

Description of key information

The microbial growth was evaluated using the tetrahymena pyriformis population growth assay. The test was conducted in the static conditions for the period of 48 hours. Based on the outcomes the median inhibitory growth concentration was determied to be 116.16 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
116.16 mg/L

Additional information

Data available from various sources for test chemical and read across chemical selected on the basis of structual similarity has been reviewed to determine the toxicity of the test chemical on microorganisms. The studies are as mentioned below:

Data from the peer reviewed journal outlines that, The microbial growth was evaluated using the tetrahymena pyriformis population growth assay. The test was conducted in the static conditions for the period of 48 hours. Based on the outcomes the median inhibitory growth concentration was determied to be 116.16 mg/L.

Another study from the secondary source, summarizes the effect of test chemical on the microregma sp. a ciliated protozoan. Based on the feeding behaviour the effective concentration was determied to be 70 mg/L.

The effect of the test chemical to aquatic micro organisms was in a range of 70- 116.16 mg/L.