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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No GLP and no specified test guideline

Data source

Referenceopen allclose all

Title:
No information
Author:
Dorothy
Year:
1986
Bibliographic source:
Dorothy, A.C. et al., Mutat.Res., 172, 105-138 (1986)
Title:
No information
Author:
OECD
Year:
1999
Bibliographic source:
OECD SIDS for glycidyl methacrylate, December 1999

Materials and methods

Test guideline
Guideline:
other: bacterial reverse mutation assay
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2,3-epoxypropyl methacrylate
EC Number:
203-441-9
EC Name:
2,3-epoxypropyl methacrylate
Cas Number:
106-91-2
Molecular formula:
C7H10O3
IUPAC Name:
oxiran-2-ylmethyl methacrylate
Details on test material:
Produced by Aldrich, purity 98%

Method

Species / strain
Species / strain / cell type:
other: Salmonella typhimurium TA97, TA98, TA100, TA1535
Metabolic activation:
with and without
Metabolic activation system:
S9 from hamster liver, induced with aroclor 1254-induced, or rat liver induced with aroclor 1254
Test concentrations with justification for top dose:
0, 10, 33, 100, 333, 1000 micogram/plate
Controls
Positive controls:
yes
Positive control substance:
other: With metabolic activation: 2-aminoanthracene (all strains) Without metabolic activation: sodium azide (TA 100, TA 1535), 9aminoacridine (TA 97), 4-nitro-o-phenylenediamine (TA 98)
Details on test system and experimental conditions:
Number of replicates: once at least.
Plates/test: 3
Statistics:
Not reported

Results and discussion

Test results
Species / strain:
other: Salmonella typhimurium TA97, TA98, TA100, TA1535
Metabolic activation:
with and without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
other: unknown
Additional information on results:
Glycidyl methacrylate showed positive result in TA97, TA100, TA1535 with and without metabolic activation but not in TA98.
Conclusions: Bacterial gene mutation is positive with and without metabolic activation.
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

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