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Toxicological information

Skin sensitisation

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Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Referenceopen allclose all

Title:
No information
Author:
Dow
Year:
1992
Bibliographic source:
The Dow Chemical Company, unpublished report (dermal sensitization) (1992)
Title:
No information
Author:
OECD
Year:
1999
Bibliographic source:
OECD SIDS for glycidyl methacrylate, December 1999

Materials and methods

Test guideline
Guideline:
other: Delayed allergy reaction test
GLP compliance:
not specified
Type of study:
Buehler test

Test material

Constituent 1
Chemical structure
Reference substance name:
2,3-epoxypropyl methacrylate
EC Number:
203-441-9
EC Name:
2,3-epoxypropyl methacrylate
Cas Number:
106-91-2
Molecular formula:
C7H10O3
IUPAC Name:
oxiran-2-ylmethyl methacrylate

In vivo test system

Test animals

Species:
guinea pig
Strain:
Hartley
Sex:
male

Study design: in vivo (non-LLNA)

Induction
Concentration / amount:
0.4 ml of a 10% or 25% solution of glycidyl methacrylate in dipropylene glycol monomethyl ether (DPGME).
Challenge
Concentration / amount:
0.4 ml of a 10% or 25% solution of glycidyl methacrylate in dipropylene glycol monomethyl ether (DPGME).
Details on study design:
10 animals received three topical applications on the left side with 0.4 ml of 10 or 25 % glycidyl methacrylate in dipropylene glycol monomethyl ether (DPGME) during the three week induction phase. The animals with slight erythema on the dermal test site after the second application received the third application at lower concentration. The applications were removed after a six-hour exposure period. After a two-week rest period, the single challenge application was conducted to the right side of the animals in the same manner as the induction applications. The animals with slight erythema on the dermal test site after the third induction exposure received a 1 % solution of glycidyl methacrylate in DPGME. As positive control (10 animals), DER 331 epoxy resin in DPGME was used.
Positive control substance(s):
yes
Remarks:
DER 331 epoxy resin

Results and discussion

In vivo (non-LLNA)

Resultsopen allclose all
Reading:
1st reading
Group:
test chemical
Dose level:
10% and 25%
No. with + reactions:
7
Total no. in group:
10
Clinical observations:
slight erythema
Remarks on result:
other: Reading: 1st reading. Group: test group. Dose level: 10% and 25%. No with. + reactions: 7.0. Total no. in groups: 10.0. Clinical observations: slight erythema.
Reading:
1st reading
Group:
positive control
Dose level:
10% and 25%
No. with + reactions:
8
Total no. in group:
10
Clinical observations:
slight erythema
Remarks on result:
other: Reading: 1st reading. Group: positive control. Dose level: 10% and 25%. No with. + reactions: 8.0. Total no. in groups: 10.0. Clinical observations: slight erythema.

Applicant's summary and conclusion