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EC number: 202-617-2
CAS number: 97-90-5
Increases in the percentage of aberrant cells were
observed at 100, 400, and 600 mg/l in male donor cultures at 72 h sampling time and in female donor cultures at 600 mg/l harvested after 72 h; no significant increases were observed
at 96 h sampling time.
cytogenetic assay (Chromosome aberration test with human
lymhocytes from two donors (1 f, 1 m) according to OECD 473) human
exposed to Ethylene glycol dimethacrylate (purity: 98 % w/w,
solvent: DMSO) at
concentrations in the range of 25 to 5000 µg/mL
presence and absence of mammalian metabolic activation S9 -mix
(Aroclor 1254 induced rat liver homogenate).
assay was performed in two independent experiments using
Ethylene glycol dimethacrylate (EGDMA) concentrations of 25,
100, 200, 400, 600 and 800 µg/mL in the presence of S9 -mix
and concentrations of 100, 500, 1000, 2000 , 3500 and 5000
µg/mL in the absence of S9 -mix. Dose related reductions in
mitotic activity were observed in cultures from both donors in
the presence and absence of S9 -mix at the 72 hour sampling
treated with higher concentrations of EGDMA (600 µg/mL -S9
-mix and 1000 µg/mL +S9 -mix) in the main cytogenetic tests
were considered not to be suitable for chromosomal aberration
analysis due to lack of metaphases as a result of toxicity or
due to cytotoxic effects on the chromosome structure.
from both donors exposed with EGDMA at concentrations of 100,
400 and 600 µg/mL in the absence of S9 -mix and 100, 500 and
1000 µg/mL in the presence of S9 -mix were choosen for
chromosomal aberration analysis at 72 hours sampling time.
Additionally, cultures from the female donor treated at 400
µg/mL in the absence of S9 -mix and 1000 µg/mL in the presence
of S9 -mix were selected for analysis at 96 hour sampling
statistically or biologically significant increases in
percentage of aberrant cells, compared to the solvent control
values, were seen at any of the EGDMA concentrations tested in
either donor, in the presence of S9 -mix at the 72 hour
sampling time or in the presence or absence of S9 -mix at 96
hour sampling time. Statistically significant increases of
chromosomal aberrations were seen in cultures from both donors
treated with EGDMA at 600 µg/mL in the absence of S9 -mix and
examined at 72 hours sampling time.
increases were observed at the 96-hours.
controls (mitomycin C and cyclophosphamide) induced the
appropriate response confirming the sensitivity of the test
Therefore EGDMA showed under the
experimental conditions reported reproducibly induced chromosomal
damage in human peripheral blood lymphocytes in vitro in the
absence of auxiliary mammalian
metabolic activation S9 -mix.
is classified as acceptable. This
study satisfies the requirement
for Test Guideline OECD 473 for in vitro cytogenetic mutagenicity
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