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EC number: 202-617-2
CAS number: 97-90-5
Mean incidence of micronucleated polychromatic erythrocytes/1000
polychromatic erythrocytes ± standard deviation (SD) at two
incidence of mean incidence of
MPE/1000 PE ± SD MPE/1000 PE ±
mean animal data mean animal data
24h 48h 24h 48h
11 vehicle control 10mL/kg 0.6 ±0.6
0.6 ±0.6 0.8 ±0.8
12 Cycolphosphamide 65mg/kg 20.6 ±4.5**
13 EGDMA 1250mg/kg 0.6 ±0.6
0.8 ±0.5(4) 1.2 ±1.1
PE= polychromatic erythrocytes
MPE= micronucleated polychromatic erythrocytes
SD= standard deviation
** statistically significant increase in micronucleated polychromatic
erythrocytes at p<0.01 in the Student's 't' test (one-side) on
All means based on 5 animals except where indicated in parentheses.
Mean percentage of polychromatic erythrocytes ± standard deviation
(SD) at two sampling times:
group compound dose mean
% of polychromatic mean % polychromatic
± SD erythrocytes ±
animal data mean animal data
11 vehicle control 10mL/kg 54.1 ±17.0
49.6 ±10.3 52.4 ±8.3 48.0 ±13.8
12 Cycolphosphamide 65mg/kg 40.4 ±4.9*
13 EGDMA 1250mg/kg 51.7 ±15.5
39.0 ±8.8(4) 49.1 ±7.7 54.6
* statistically significant decrease in the percentage of polychromatic
erythrocytes at p<0.05 in the Student's 't' test (one-sided).
In a CD-1 mouse bone
marrow micronucleus assay, 5 animals/male/female/dose were treated
orally with Ethylene glycol dimethacrylate (98.8%,
stabilizied) at a single dose of 1250 mg/kg bw. The test article was
suspended in corn oil. This suspending agent was used as negative
control. The volume administered orally was 10 ml/kg b.w.. 24 h and 48
hours after a single application of the test article the bone marrow
cells were collected for micronuclei analysis. Ten animals (5 males
and 5 females) per test group were evaluated for the occurrence of
micronuclei. 1000 polychromatic erythrocytes (PCE) per animal were
scored for micronuclei.
To describe a cytotoxic
effect due to the treatment with the test article the ratio between
polychromatic and normochromatic erythrocytes (NCE) was determined in
the same sample and reported as the number of NCE per 1000 PCE.
The following dose level
of the test article was investigated:
24 h and 48 h preparation
interval: 1250 mg/kg b.w..
In a pre-experiment this
dose level was estimated to be the maximum attainable dose. After
treatment with the test article the ratio between PCEs and NCEs was
not affected as compared to the corresponding negative control thus
indicating no cytotoxic effects.
In comparison with the
corresponding negative control there was no enhancement in the
frequency of the detected micronuclei at any preparation interval
after application of the test article.
An appropriate reference
mutagen was used as positive control which showed a distinct increase
of induced micronucleus frequency.
This study is classified
as accceptable. This study satisfies the requirement for Test
Guideline OECD 474 for in vivo cytogenetic mutagenicity data.
glycol dimethacrylate is considered to be non-mutagenic in this
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